Abstract:Damage and functional disorder in human tissues/organs could be treated by cell transplantation.However， the functional cells for transplantation isolated in vivo are limited by the source and amount. How to obtain the suitable functional cells in vitro for treatment became the key question here. Currently， lots of important progresses have been achieved in producing the functional cells in vitro. The review mainly focuses on the progress and prospect of the functional cells differentiated from embryonic stem cells/induced pluripotent stem cells or transdifferentiated from other somatic cells， which may promote the further study and clinical transformation.