Abstract:Non-coding RNAs refer to RNA molecules not being translated into proteins， but function at the RNA level. Non-coding RNAs could modify gene expression either transcriptionally or post-translationally， thus play essential roles in epigenetic regulation. Besides non-coding RNAs， the N 6-methyladenosine (m6A) of adenosine also regulates posttranscriptional gene expression. Increasing lines of evidence have shown that both non-coding RNAs and m6A modification are key factors for stem cell pluripotency and cell fate control. Here we will introduce the achievements made by the RNA research group during the supporting period of the “Stem Cell and Regenerative Medicine” Strategic Priority Research Program of the Chinese Academy of Sciences， especially on the functional studies of non-coding RNAs and m6A modification on stem cell pluripotency and cell reprogramming.