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Application of aptamer nanobiosensor in penicillin detection

适配子纳米生物传感器在青霉素检测中的应用



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ChineseJournalofBioprocessEngineering
Vol.9No.4
Jul.2011
doi:10.3969/j.issn.1672-3678.2011.04.013
OPQI
:2011-05-03
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Applicationofaptamernanobiosensorinpenicilindetection
SUNKun,ZHANGJuankun,XUPeidong,WANGMingwei,LINQing,XUJinfeng,SONGKe
(KeyLaboratoryofIndustrialMicrobiologyoftheMinistryofEducation,TianjinKeyLaboratoryofIndustrialMicrobiology,
ColegeofBioengineering,TianjinUniversityofScienceandTechnology,Tianjin300457,China)
Abstract:Poroussilicon(PS)wasmadefromsiliconsbyapulsedcurentetchingmethod.Threemeth
odsweretestedtochoosethebestwayofimmobilizingpenicilinaptameronglasssheet,caledthecova
lentbondingmethod,ionabsorbingmethod,andAPTESmodifiedglutaraldehydecrosslinkingmethod.
AfterpenicilinaptamerwasimmobilizedonthemodifiedPS,theimpedancesweredeterminedandcom
paredbyACimpedencemethodunderconditionsofaddingandwithoutaddingpenicilin.Then,an
equivalentcircuitwasestablishedforimpedancefiting.TheNyquistplotsofPSsensorcouldprovethat
theaptamerwasimmobilizedandthenanobiosensorestablished.Thelinearrangeofthesensorwas
005-020mg/L.Thesensitivitylimitwas005mg/L.
Keywords:aptamer;biosensor;poroussilicon
  
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