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Lipase-catalyzed synthesis of cinnamyl acetate in organic solvents

有机相脂肪酶催化合成乙酸肉桂酯



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ChineseJournalofBioprocessEngineering
Vol.10No.3
May2012
doi:10.3969/j.issn.1672-3678.2012.03.003
OPQI
:2011-11-09
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:1672-3678(2012)03-0012-05
Lipasecatalyzedsynthesisofcinnamylacetateinorganicsolvents
YAOXia,ZHENGJianyong,YINGXiangxian,SHENXueliang,WANGZhao
(ColegeofBiologicalandEnvironmentalEngineering,ZhejiangUniversityofTechnology,Hangzhou310014,China)
Abstract:Enzymatictransesterificationofcinnamylalcoholwithvinylacetateinorganicsolventswas
studied.LipasefromCandidaanatarctic,Rhizopusniveus,andPseudomonasfluorepossessedgoodcata
lyticactivity.TheefectsofthereactionparameterscatalyzedbyNovozyme435weresystematicalyinves
tigatedandtheoptimumoperationswereobtainedasfolows:cinnamylalcohol200mmol/L,cinnamylal
cohol/vinylacetate(mol/mol)ratio1∶15,initialwateractivityαw =084,reactiontemperatureof
30℃,with002glipasein10mLmethyltertbutylether.Undertheaboveoptimumconditions,the
maximumreactionconversionreached99% after3h.Theproductwasidentifiedbymassspectory(MS).
Thelipasewasstablewhenreusedfor10times,andthereactionconversionwasabove90%.
Keywords:cinnamylacetate;lipase;transesterification;cinnamylacohol;vinylacetate
  
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[1] 
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,2008.
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101260042[P].2008-09-10.
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úû/Þ

üv~

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