Abstract:The Ferula ferulaeoides seeds were irradiated using different dose of nitrogen ions with 30 KeV energy for studying the responses of resistance physiological indexes. The objective is to provide foundation to protect the ephemeral plant Ferula ferulaeoides in arid areas. Results showed that the irradiation with high dose of nitrogen ions significantly inhibited germination rate and germination index. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase(CAT) were enhanced with the increase of nitrogen ions. However, excessive nitrogen ions irradiating reduced the activities of SOD, POD and CAT. The peroxidase proline content fluctuated with increase of nitrogen ions irradiation. First it decreased, then increased and reached the peak at dose of 1×1016 N+/cm2, and then decreased. Besides, the soluble protein content significantly increased with the increase of nitrogen ions irradiation, reached the peak at doses of 6×1016N+/cm2, then decreased. By analyzing the variance of germination rate and germination index, the conclusion could be drawn the optimum dose of nitrogen ions irradiation could break seed dormancy, improve germination and enhance the activities of protective enzymes and content of proline and soluble protein.