摘 要 ：为探讨不同生态区烟叶香气风格形成的机理，应用蛋白质双向电泳联用质谱技术，对河南平顶山（浓香型烟叶的典型生态区）和福建龙岩（清香型烟叶的典型生态区）的烟草（Nicotina tobaccum L. cv. K326）叶片蛋白质组成进行了比较研究。结果发现，51个蛋白质在两个生态区发生了差异表达，其中在河南表达量上升的有15个，在福建表达量上升的有25个。另外，还分别有2个和9个蛋白点为在河南和在福建样品中特异表达。采用MALD I-TOF/MS进行肽质量指纹图谱分析，经MSDB、NCBInr和SwissPort数据库查询,共鉴定出25种蛋白质，其中参与叶绿体发育、色素代谢、光合作用相关的蛋白在福建烟区高表达，与糖酵解途径相关的蛋白质在河南烟区中高表达。另外， 在两生态区烟叶中都发现有相当数量的特异表达的抗逆和防御蛋白。首次在蛋白质组学水平对不同香气风格烟叶的形成机理进行了探讨。
Abstract:To explore the molecular mechanism of tobacco aroma style formation, protein profiles of mature leaves of tobacco (Nicotina tobaccum L. cv. K326) plants grown respectively in Henan province (Central China) and Fujian province (South China), were compared using two-dimensional electrophoresis. Fifty-one proteins were found to be differentially expressed between tobacco plants from the two different ecological regions. Among them, 15 proteins were up-regulated and 2 were specific to Henan tobacco leaves, while 25 proteins were up-regulated and 9 were specific in Fujian tobacco leaves. Next, the differentially expressed proteins were subjected to MALDI-TOF analysis for identification. It turned out 25 proteins matching homologous sequences in MSDB and NCBInr database. In particular, several proteins involved in chloroplast development, pigment metabolism, photosynthesis were up-regulated in Fujian tobacco leaves, while proteins involved in glycolytic pathway were relatively abundant in Henan tobacco leaves. In addition, some specific proteins related to stress responses were identified both in Henan and Fujian tobacco plants. Thus, the different expression patterns of leaf proteomes may contribute to the formation of special tobacco aroma style. To our knowledge, this was the first attempt to explore the mechanism of tobacco aroma style formation on a proteome level.