Abstract:A laboratory soil incubation experiment, was conducted using soil from a wheat field in Shaanxi Province,to study, the influence of wheat roots soil moisture and temperature on N2O emission from soil. The results indicated that. N2O flux was higher in soil containing wheat plants compared to unplanted (fallow) treatments during the entire growth period. Maximum N2O flux occurred at the booting stage, and then decreased as root activity decreased in later growth stages. N2O emission in the main root-zone and the inter-row was different. The largest differences occurred at the booting stage, when the flux of N2O between plants was 5.64 times more than in the main root-zone. There was no difference in N2O emission form planted and unplanted treatments at flowering and maturity. During the latter two stages, temperature effects due to wheat roots had a significant effect on N2O emission. Moisture effects due to roots were significant in the 15-20cm layer. The results also indicated that N2O increased due to the activity of wheat roots in the soil.