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作 者 ：王通, 梁炫强, 王冕, 陈小平, 潘丽娟, 陈明娜, 陈娜, 迟晓元, 禹山林

期 刊 ：核农学报 2016年 30卷 3期 页码：437-443

关键词：花生；GLP家族基因；组织表达；干旱；

Keywords:peanut (Arachis hypogaea.L), GLP family genes, tissue expression, drougth stress,

摘 要 ：为深入探索花生GLP家族基因的功能,利用实时荧光定量PCR技术比较不同花生品种种皮和干旱胁迫下花生不同组织中花生GLP家族基因的表达情况。结果表明,花生GLP家族基因在粉红色花生品种种皮中的表达量显著高于其它颜色种皮;在花生种皮中,AhGLP3和AhGLP6的表达量相对最高,其次是AhGLP1、AhGLP2和AhGLP8。正常生长条件下,除AhGLP2和AhGLP8,其它各基因在种子、根和叶中的表达均有明显的组织差异。在干旱胁迫下,AhGLP1在叶片中的表达量发生上调,而AhGLP3、AhGLP4和AhGLP5在种子和根中的表达量显著上调;AhGLP2、AhGLP7和AhGLP8在各组织中均受干旱诱导而表达量上调,而AhGLP6的表达量下降。结果表明花生GLP家族基因的表达有明显的品种特异性、组织和诱导差异性。本研究为阐明花生GLP家族基因的组织及抗逆表达提供理论基础。

Abstract:In order to study the function of the peanut GLP family genes, differential expression of eight AhGLPs between peanut testa, seed, leaf and root was analyzed under drought condition. Results showed that expression of the eight AhGLPs were higher significantly in pink testa than in others. Moreover, in peanut testa, AhGLP3 and AhGLP6 expressed highest, and followed by AhGLP1, AhGLP2 and AhGLP8. Under drought stress condition, AhGLP1 was induce-expressed only in leaves. AhGLP3, AhGLP4 and AhGLP5 were up-regulated significantly in seeds and root, and the expression of AhGLP2, AhGLP7 and AhGLP8 were induced in root, seeds and leaves by drought stress. On the contrary, AhGLP6 was down-regulated by drought stress. Under the condition of normal growth, AhGLP1, AhGLP3, AhGLP4, AhGLP5, AhGLP6 and AhGLP7 were expressed with tissue-specificity. The above results indicate that the expression of peanut GLP family genes have species- and tissue-specificity under different conditions. It could provide valuable information for the fuctional and regulatory stydies on peanut AhGLPs genes especially under drought stress.

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