Abstract:To discuss and study the regulation effect of biological-sugar fertilizer and urea on soybean nodule, Hefeng55 was used as the test material to research the effect of different types of nitrogen on soybean nodule number, nodule dry weight, root dry weight, aboveground dry weight and effective nodule. Four treatments were applied at random block design, marked with N1 (150 kg·hm-2 biological-sugar fertilizer), N2 (225 kg·hm-2 biological-sugar fertilizer), N3 (150 kg·hm-2urea), and N4 (75 kg·hm-2 urea). The results showed that the effect of different treatments on soybean seed yield followed the order of N1>N2>N4>N3 and yield difference among four treatments was significant. Seed yield under N1 treatment was 6.24%, 21.57%, 9.39% higher than that under N2, N3 and N4, respectively. Nodule number, dry weight of root nodule, root dry weight, aboveground dry weight and effective nodule under N1 treatment were highest, and N1 treatment got the highest seed yield. The result indicated that biological-sugar fertilizer promoted the growth and development of soybean nodule and therefore increased soybean seed yield. Results obtained from this experiment will provide theoretical reference for nitrogen nutrition regulation and fertilization of high-yield soybean.