Abstract:To breed new peanut variety with high-oleic and high-yield, a hybridized combination was made between 06-I8B4 (high sucrose, female parent) and CTWE (high-oleic, male parent). Using molecular marker technology coupled with NIRS (Near infrared reflectance spectroscopy) assisted selection, Huayu 662, a new high-oleic peanut variety with FAD2A/FAD2B double mutation was finally developed. The seed of Huayu 662 contained 80.8% oleic acid in its total fatty acids. The oleate/linoleate ratio of the cultivar was as high as 29.92. The shelling outturn was 79.7%. In 2012, Huayu 662 produced a kernel yield of 5 132.42 kg·hm-2, comparable to the control Huayu 33 output of 5224.5 kg·hm-2 in yield test on the SPRI Experimental Farm in Laixi, Qingdao, China. The cultivar was approved for release in Anhui province for commercial production in 2014. The research provided a good technical solution to speed up the breeding process of new high-oleic peanuts..