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作 者 ：鲁黎明, 陈勇, 鲁逸飞, 李立芹

期 刊 ：核农学报 2016年 30卷 7期 页码：1273-1280

关键词：烟草；低钾；基因表达谱；非生物胁迫；

Keywords:tobacco, low potassium, gene expression profiling, abiotic stress,

摘 要 ：为探索在低钾胁迫下烟草幼苗基因表达谱的变化,对烟草幼苗进行了0、6、12、24 h低钾胁迫,并利用基因芯片技术分析了烟草幼苗基因表达谱的变化。结果表明,与对照相比,处理组基因表达量变化在2倍以上的差异表达基因共有3 790个。这些差异表达基因所行使的功能多达18种,包括抗氧化活性、催化活性、酶调活性,并参与应激反应、发育过程以及代谢过程等。在差异表达基因中,涉及到响应非生物刺激相关差异表达基因有417个(281个上调、136个下调)、光合作用基因有21个(10个上调、11个下调)、光合色素合成相关基因9个(6个上调、3个下调)以及植物激素代谢相关基因6个(5个上调、1个下调)。研究结果可为烟草钾营养的分子机制研究提供借鉴。

Abstract:In order to explore changes of gene expression profiles of tobacco seedlings under low potassium stress, tobacco seedlings plants were treated with low potassium stress for 0, 6, 12, 24h and gene expression profiles of tobacco seedlings at each time point were analyzed with genechip. The results showed that compared with control, a total of 3 790 genes whose expression levels changed more than two times were detected. GO analysis showed that these differentially expressed genes can be divided into 18 functional classifications including antioxidant activity, stress response, the catalytic activity, development process, metabolism, biological regulation, and etc. Of the 3 790 genes, 417 were involved in non-biological stimuli related genes (281 up-regulated, 136 down regulated), 21 photosynthesis related genes (10 up-regulated, 11 down-regulated), 9 photosynthetic pigment synthesis related genes (6 up-regulated, 3 down-regulated) and 6 plant hormone metabolism related genes (5 up-regulated, 1 down-regulated). This result indicates that low potassium stress induces significant changes of expression levels of many genes including photosynthesis, and may impact the photosynthesis and development of tobacco seedlings. This study lays foundation for the research of molecular mechanisms of tobacco potassium nutrition.

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