Abstract:To explore exogenous EBR‘s relief of soybean seedlings Cd poisoning mechanism, soybean variety Hei Nong 48 was employed as the test material and treated under nutrient solution culture with different concentrations (0.01,0.05,0.1,0.2,0.5mg·L-1 ) 24-epibrassinolide (24-epibrassinolide, EBR) process soybean seedlings under 10mg·L-1 Cd stress, to investigate the effects of exogenous EBR on the height and root growth, peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) three kinds of antioxidant enzyme activities, malondialdehyde (MDA) content, chlorophyll content of soybean seedlings under Cd stress. The results showed that under Cd stress, not significant difference was observed between spraying 0.01 to 0.1mg·L-1EBR on soybean growth index and the Cd stress alone , while the treatment with high concentrations of EBR spraying 0.2 to 0.5mg·L-1 the optimum concentration range EBR could significantly increase plant height, root length, fresh weight underground, underground dry weight, compared with Cd stress alone increased by 17.9%, 17.2 %, 33.3% and 38.1%, respectively. Low concentration EBR (0.01 and 0.05mg·L-1) did not significantly improve three kinds of antioxidant enzyme activities and chlorophyll content or decrease MDA content not, in (0.1 to 0.5mg·L-1) spraying the optimum concentration range EBR, after treatments of 2d and 8d stress make POD, CAT, APX activities and chlorophyll content than Cd treatment alone increased 42.6% and 48.1%, 33.4% and 67.7%, 116.8% and 64.8%, 29.7% and 39.1% , while MDA concentration decreased by 19.7% and 27.8% than the individual Cd treatment. Thus, 0.1 to 0.5mg·L-1EBR was more appropriate spraying concentration, and the 0.5mg·L-1EBR was the optimal spray concentration. Appropriate concentration of exogenous EBR could promote the growth of soybean under Cd stress, improve the antioxidant enzyme activities, reduce the degree of membrane lipid peroxidation and photosynthetic system damage, was conducive to enhancing the capacity of plant resistance to Cd stress. This study to elucidate the role of EBR relieving the injury of soybean seedlings under Cd mechanism provides the theoretical basis.