Abstract:In this study, experments with tissue culture seedlings of two-year-old Catapla bungei clones (015-1,7080,1-4 and 004-1) were conducted to explore the seedlings’ characteristics of absorption, utilization and allocation of nitrogen. By using the stable isotope15N tracer, we measured the absorption, nitrogen use efficiency (NUE) and allocation rates were measured under an exponential fertilization trial. The results were as follows: the nitrogen absorption, NUE and allocation rates of the five C. bungei clones all exhibited a high consistency, with NUE ranging from 27.14% to 31.24%. The NDFF of roots and leaves of C. bungei clones were significantly higher than stems. The roots and leaves were more competitive than stems for nitrogen fertilizer. The nitrogen allocation rate in the stem of C. bungei clone 015-1, as same as in the root of C. bungei clone 7080, were significantly higher (p<0.05) than the other clones. There was significant difference (P<0.05) among the nitrogen allocation rates in the three organs. In general, the allocation rates in the three organs followed the order of leaves roots stems. These findings provide a scientific basis for rational application of nitrogen fertilizer of C. bungei.