Abstract:For further improving the efficiency of wheat anther-culture breeding and broadening the genotypes of crossing parents potentially applited in anther culture, the anther culture characteristics were evaluated and analyzed with 86 Gansu wheat cultivars and backbone parents by using anther culture technology; the application potential were discussed in the screened high-anther-culture-ability genotypes. The results indicated the spring wheat genotypes were easier to be inducted to form callus and then differentiate into green plantlets than that of winter wheat ones in this study; there was no correlation between the callus induction frequencies and green plantlet or albino plantlet differentiation frequencies; the green plantlet production frequency could be used as an important index in evaluating anther culture ability. The study will not only provide reference for wheat anther-culture breeding in using Gansu wheat germpalsm, but the selected high-anther-culture-ability genotypes are of important potential application value in wheat anther culture breeding, DH population establishment, microspore culture, and fundamental research.