免费文献传递   相关文献

THE ASSESSMENT OF GENETIC DIVERSITY OF PEAR CULTIVARS FROM SOUTHERN AREA  OF ZHEJIANG PROVINCE BY USING SSR AND AFLP ANALYSIS

浙江省南部地方梨品种的SSR和AFLP遗传多样性研究



全 文 :!! " # $
!"#$#!"%""#$"%( "%*
!"#$%&"()#*+&$,-$.*#/#$&0*.+%*+1
!"#$
$$###)*++$""#$###")#"%()#,
žs9¡©àxS*1
--2
˜
Gi:.
opÕî¥67
no=
$
!
pqr
"
!
.
!
(
(
!
sSt
$
!
uv5
$
"$/
ž;#‹Ýß
!
":¡‹Ý>?¢u¦)RS­™(MN:j%/g
!
ž yJ
!($##"&&
"/
fQz:DE#‰^_Rìjß
!
ž fQ
!("(###&(/
žsE{–‹¡DEß
!
ž E{
!($1+###
%
!
&
$
e‘ÅÆÇÈÉʅ9Ë*+-‚†7
!
d"
$#
0ÌN4
(
0Ë"-
--2
ˆ[_`
,
0
Gi:.
ˆ[0͚ÒÎ4”…-
$*
…9Ë*+¹ŒsEF‘+KL

MNOP
$#
0ÌNˆ[v
Ë"&J|
,"
åå‰
$(
0
--2
ˆ[-ÏÐô4×
"E+#
e
#/#+, #/&%%!
#/+1!
‡Ñ5’
¬
"A#
-“”
#/(%# "/#$(!
$/"" ,
0
Gi:.
ˆ[ÒµŒ&J|
(,%
å‰
!
r‘0ˆ[&
J
,#/,1
å
!
r‘·7–—e
1(/(+0
d"
4.AgG
£ŒKLf,--
--2
4
Gi:.
,ÓԚ
!
Õ

%
Õ4¿ÉÖË»Õ4×ÉÖËv
"
,ÓԚ£vÅØ
!
ÙVžÚRkW^-‚ž•×
!
ÛÜ
˜t£-*+
&(
£óeÝÖË-*+
"
ÝÖË
$%
ÝÖË
"
4ÝÖË
(#
KÞvt£-Қ
!
e†
[£ó
--2
4
Gi:.
£ŒMN—=ßµ
!
Û
Gi:.
-MNà´µ*+-…áK¨â‡

()
$
ÒÎ
&
”…
&
…9Ë*+
&--2&Gi:.&
‚†7
*+,-
$"##&)#1)#,!
./,-
$"##&)$#)",
0123
$
žs¹áY#ÄI‚±,Êþ|OP
"2(#1,#+#!
žs
"##*
Ê8YDKL
""##*J("#$$#
4567
$
l5ƒ
"$&*")#!
t
!
žI8k
!
¨«”m¢
!
67àÞû±eopÕî¥
C6X$#+1$)*,&1$*#(& K[D9X$ NWQD9#%^$,(/Q=[
8945
$
}†ø
"$&,()#!
g
!
~Jk
!
”m
!
xy
!
”m¢-“
!
67àÞû±eìjRL…)*
C6X$#+1$)*,&1$*#(& K)[D9X$ WTS6>?^
7Y@/6N@/Q>
#J&$++&++!&#,*%&&#().(F&-+(#0,*E&$-)"1#(F$-+*-,! +,"#J&-$-&$
,*WJ&V($%E-,F()&/0"+(%++-$.$*1E$$10+(+
JG;5D>)W9>?
$
!.GHE89)[69
"
!3GHACD=
(
! EFKHAj9D=)WD>
$
!CKHA<@D>)T6>
$
"$/U+3&$/C+%/"(E"$/.*#/#$+! Q4+L.&%- :%.;+$1./U+;+"3C+%/YYH#&./4"#! Q4+L.&%-!($##"&&
"/J.14#.N"*&/."%&YI+*4%.*&9"+-+! J.14#.! Q4+L.&%-!("(###& (/S+%.%- 7T/+%1."% 0/&/."% ("$03+*.&J"*&2$"6#*/1! S+%.%-! Q4+L.&%-!($1+## #
$2345674$ GS=SDX=P$* U6DMQ@XS9RDMO=MSWU6O>DS9R6S=36>X9>?D>N :9O8@99> S86O=@S86M> E86Y9D>?.M=R9>Q6=PJ89>D
T6M6@O6N S=DOO6OOS869M?6>6S9QN9R6MO9SWVW, Gi:."G[UX9P96N iMD?[6>S:6>?S8 .=XW[=MU89O[# UM9[6MQ=[V9>DS9=>O
D>N $( UD9MO=P--2"-9[UX6-6L@6>Q626U6DSO# UM9[6MO! =PT89Q8 $# UD9MOT6M6PM=[DUUX6D>N ( UD9MOT6M6PM=[
U6DM/C86M6O@XSO9>N9QDS6N S8DS," DX6X6OT6M6N6S6QS6N VW$# UD9MO=P--2UM9[6MOPM=[DUUX6/C86MD>?6=PS86
=VO6MR6N 86S6M=7W?=O9SW9> $( UD9MO=P--2UM9[6MOTDOV6ST66> #/#+, D>N #/&%% T9S8 D> DR6MD?6=P#/+1! D>N S86
MD>?6=P-8D>>=> 9>P=M[DS9=> 9>N6kTDOV6ST66> #/(%# D>N "/#$( T9S8 D> DR6MD?6=P$/""/GV=@S1( U6MQ6>S=P(,%
VD>NOUM=N@Q6N VW@O9>?, Gi:.UM9[6MQ=[V9>DS9=>OT6M6U=XW[=MU89Q/]DO6N => S864.AgGQX@OS6MD>DXWO9O=P--2
D>N Gi:.NDSD! 1<@>86X90! 1<@>86J@8@Dk@6X90 D>N 1<@>86j98@Dk@6X90 T6M6Q=[U=O6N =PDO@V?M=@U T9S8 D
M6XDS9R6XW89?8 O9[9XDM9SW! V@SS86WV6X=>?6N S=S86N9P6M6>SQ@XS9RDMO/1:D=k@6X9$0! 1:D=k@6X9"0 D>N 1:D=k@6X9(0
8DN S86OD[6>D[6! V@ST6M69> N9P6M6>S?M=@UO! 9>N9QDS9>?S8DSS86WT6M68=[=>W[W/C86N6>NM=?MD[PM=[--2D>N
Gi:.?6>6MDXWD?M66N T9S8 6DQ8 =S86M!=PT89Q8 S86Gi:.M6O@XSOT6M6[=M69> DQQ=MND>Q6T9S8 ?6=?MDU89QN9OSM9V@S9=>/
89: ;<5=3$36>X9>?& :9O8@9& X=QDXU6DMQ@XS9RDM& --2& Gi:.& ?6>6S9QN9R6MO9SW
(%"
!!
"
!
#
!
$
"%
Ü
!!
žs|Í"2<$#1:/#
>?1_¡©F
W
!
L‡¶€x
"2/3<$.("./HD\D9#%
§ x
"2/
*&+$<&%& 5Q>6#%
,x¶"2/#1#$.+%1.1gDk9[/
RDM/4"%6"+%1.126XN/#
˜Yx‚
*$+
!
_Dƒ°S*‰Í
û€xßQ
!
Pþ;ض
"#
֝ž_¡€xS*Œ¿
^Ǩ<=¶`*­ìjVF

žspQ1©àS
*‰ÍL‡¨žF¡ÿ9¡©®
*$+

7ÊÌ÷å
¶&¨fQ˜E{‚©r@xìj™š3¦½×WØ
‚d¨¸]´¶ý<=*­ìjVŒ¿^Ç1xS*
˜ÙÚ

띞s":Y#‰‹Ý67þWØ©à
S*r@×Tu#™šð
*$+
!
PþþçØ©àS*1
67ì¯Õð

û×=c©V՘}~çØ©àS
*
!
ƒ„©àS*F»Û*­1Îi
!
1ÍþçØ©à
S*r@© ˜opÕî¥L£

5HG
—xýï·|x[>?*­ìj© ˜o
pÕî¥L£Á¶41Bé
!
‹ê|
--2
—x
"-9[UX6-6L@6>Q626U6DSO#
*"+
˜
Gi:.
—x
"
ÃÁÙD
u 8 Õ u ¥
! G[UX9P96N iMD?[6>S :6>?S8
.=XW[=MU89O[#
*(+
K0Õu¥”
%
Mô¥cΨx[>
?16â¡2
%
opÕî¥L£˜S*© ‚àªå
Ë×Hÿ15~
*% $#+
‚
*$$+
„z…±‡:
¦1
--2
$?覩5~Ë
(,
øxS*1opL£
F
!
¼·çØ
--2
$?¨…±˜x[>?F鶉~
¥
!
½Ë~0VÕx[>?1opÕî¥
3t>OQ8
˜
F=M[D7D
*&+
]~zx‡:¦1
--2
$?© ×
,(
ø
†‡xS*
!
¤F
,$
øS*ò)·¸©ý®L
! ]M9>9
‚
*%+
}~
--2
$?þ
"+
Ö¾ˆ‰©àxS*r@×
opÕî¥L£
!
ûçØ©àS*1^Ç%&×Ãú
()
]D=
‚ÏÐ}~
--2
*++
˜
Gi:.
—x
*,+
þ7
$##
ÖIexS*r@×_ê`ߘopÕî¥6
7
!"
*—xå°±Ä÷WU

cˆ(‚
*1+
˜l5
ƒ‚
**+
ºÏÐ]~
--2
˜
Gi:.
—xþ~sF¡x
*­ìjr@opÕ_ê`ß167
!
¸b×
æ©®xìjíÙ1opÕî¥

÷67}~
--2
˜
Gi:."
*—x
!
`þk¨
xopÕî¥67Fª«å$?
!
þž9¡E{
˜fQ1¡L©àxS*r@© ˜opÕî¥L
£
!
ûçØ©àx*­ìj1^˜}~%&()

$!
®¯˜à*
>?>@
:;
$*
øxîS
"
¼
$#
J%©]¹žs1E{˜
fQ©®
!
¤F;¡L2|áҌ¿˜$;

]¿
(%
%
1Ö1µY’Ù
!
VŠw^Ç0
c1#`
»Etq%
~

J
>@
mn?öÐq[YZ
CDVX6$!.6DMDQQ6OO9=>O@O6N 9> S89OOS@NW
]¿©
X6DPO=@MQ6Q=N6
G(
H=Z
S*Ý
U6DMDQQ6OO9=>
G(
H=Z
S*Ý
U6DMDQQ6OO9=>
E{©®
36>X9>? $
ˆ˜x
<@>86X9 ,
‹Gcx
E8@k9>8@D>?X9
"
Rcx
j9D=8@D>?X9 1
Œx
CD>?X9
(
x
.@S=>?X9 *
Yx
g69X9
%
,Žx
m9>?W@X9 &
Qcx
-8@98@D>?X9
+
‘©
]D9S=@O8D= $#
¥x
2@=VD=X9
fQ©®
:9O8@9 $$
‘’x
( :D=k@6X9( $+
s1’
:9@W@6k@6
$"
•‡x
E86>k9D>?X9 $,
ˆ˜Q’x
<@>86J@8@Dk@6X9
$(
‘’x
$ :D=k@6X9$ $1
‘’x
" :D=k@6X9"
$%
“”x
<9>?Q8D9X9 $*
ˆ˜ô’x
<@>86j98@Dk@6X9
>?A@
<=
$/"/$!5HG
/0
!
]~èÛ1
-5-
*
*$"+
!
zµ’F
%äĜ&
5HG!
w~Loo8K
"]6QZCD> 54*##!
4-G#

5HG
&8
!
„¤•>û
"#>?d
&
X
$/"/"!--2
-GJ‹,`Žã
!
÷./]~K
‚
*$(!$%+
zx[>?F:¦1
(
þ$?
]AC
c"(V!HF#$$V
˜
HF##1V
Ëÿz…±[>?F:¦
1
$#
þ$?
#"V$!JF#$F$#!JF#(?$"!JF#%?#%!
F9"%P#%!JH%%%1&%--2!F9"(?$"!F9#$Q$$!F9#&P#$
˜
F9""P#,
*$+ $1+
"
¼
" #
r@
.J2
ÃÁ
!
$?LêK
;>R9SM=?6>Cg
ÀȘ‡¢B¢?BCDEÆǶì
Àȍè
.J2
Ñ5ÖßÛÖ9û
"#
&
X!
¤F¥Ù
$#
e.J2]@P6M! $/+[[=Xd:g?JX
"
! #/"[[=Xd:NHC.!
‡
%
hH$?!
$#
&
[=$d:! $/#4 I&M
Ë
"CD\DMD!
IDUD>#!"#>?
1Ĝ&
5HG
.J2
Ñ5¨
KUU6>N=MP
ÀÈ1
&,
–
gDOS6MQWQX6M
AMDN96>S.J2
+‡r@
!
¤F$?
]ACc"(V
˜
HF#$$V
1Ñ5CG`MI‚
*$*+
1à*

$?
%%"
!"
È žs9¡©àxS*1
--2
˜
Gi:.
opÕî¥67
HF##1V% #"V$% JF#(?$"% JF#%?#%% F9"%P#%%
JH%%%1&%%F9"(?$"%F9#$Q$$%F9#&P#$%F9""P#,
1ÃÁ
CG|
$&%`
É3¥
+[9>&&%`

$[9>!++` ,#`
ÙÚ
$[9>!1"`
ÊË
"[9>!
>
(+
øÌ^
!
ÁÐ
1"`
Ê
Ë
,[9>!%`

*$%!$,!$1+

$?
JF#$F$#
1ÃÁC
G
$&%`
É3¥
"[9> (#O&&%`

(#O!,+`
ÙÚ
$[9>!1"`
ÊË
$[9>!
>
%
øÌ^
!
­ÒÌ^E8À»
$`!
áÐ
&%`

(#O!,#`
ÙÚ
$[9>!1"`
ÊË
$[9>!
>
(#
øÌ^
!%`

*$++

J
A@
åæçèé
--2
¯¬?÷øoôõ?ùðúû
CDVX6"!--2UM9[6MO6L@6>Q6OD>N DX6X6O976MD>?6
$?Ýí
>D[6=P
UM9[6M
$?GŽ
UM9[6MO6L@6>Q6
‚×Ĝc§
DX6X6O976
MD>?6"VU#
JF#(?$" i AJAJCAGGGGGAACJGACCC $+% "##
2 JGGAAGCAJAJGCACGCCCA
JF#%?#% i GACAAJCAGCAGAAGCAGAA $1# $*,
2 AJCGACCAJGJJGGACCJGJG
F9"%P#% i JJAGJAAJCJGGGAGJGGJ $%% $+(
2 CAGGGGACAGGAAAGGCAAGGA
JH%%%1&%--2 i JGCAAJGAACAJCGGGJCCA "(# (#,
2 ACCCAJGGJCJGJGJGGCAJGGJ
F9"(?$" i JJJCCJJJCGJJGGGCAAGJ ""( "%$
2 ACCCGGGAAAAJJJGJGGGACA
F9#$Q$$ i CCAAAJJGJCCJGJGGJGA $(* ",#
2 ACCCAGACCCAGCJCJJGGJGCCGJ
F9#&P#$ i JGJJGJJGGGCCJCJJGCJCCJ "+1 ",,
2 ACCCGJJAJJGGGCAJCCCACCGJ
F9""P#, i JGGCAAJACJCACACJGJCJ "%# "%,
2 ACCCGJAGJAAACGGAACAGCACJ
JF#$F$# i CAJGGGAGCGAACGAGCGCGCAJJG &( $$&
2 GAAGAAAGCCACCCACAJGJ
#"V$ i JJACAGCAGJGGGACAJGCAG "(*
2 GCAGACCCAGCAJJJCCAAG
]ACc"(V i JGJGCCJGGGAGCCGGAGC $&" "$+
2 GJCJGAJJCCCCCCCJJJGJ
HF##1V i CGJJCCAGCAAAGGJCAGGJ $+# "(+
2 GGCGACGAGCCAJGGCCGJCJ
HF#$$V i
CAACCJGJGCGAGAGAG)
AGAGAGAG
$,# "%#
2 CCCAJJACCAAGJJAGAJ
$/"/(!Gi:.
-GJ‹,`Žã
!
K0
Gi:.
þ
5HG
Åö1Í£ã”
!
£x’ÙF[¶ãÕ1ÕÏÿ—Ù

!
œ*]~˜C­Ðf€Œ.kn
"
‡˜C­
ĜDE¶ìÀÈ
#
þĜ&r@×ùt
Gi:.
=
nî¢üøM
*(+
w™n™4
!
~.kÿéÖ=
nî¢üøM
*,+ ,
þ$?&Lêû
$K)GGJd
g)JGA! K)GAAdg)JGG! K)GAAdg)JCC! K)GJAdg)
JCJ! K)GJGdg)JGG! K)GGJdg)JCG!
K‡¢B¢
?BCDEÆǶìÀȍè

$/"/%!.GAK

!.J2
¡?¨
,0
WÜ݉Ðf
".GAK#
Fõьv
!5HG
.†‰Š
G?HB
(
¤‘*¸
½
*,!$&+

$/"/+!
¬äù5»KL
!
g).†×ï1õ‡
!
Ë
($)
˜
(#)
Lê#¼.†1¶˜X
!
xy­ø
--2
˜
Gi:.
×NFMô¥cWùä1.†

]~
HC-<-)
UQ"/#"Y
CG
*"#+
LêK}0‚š)
" -g! -9[UX6
gDSQ8#
ßӘ
59Q6
op´ëßÓ
*"$+
!
ìåR
-g
ßÓ
´` 1 â ã
!
~ m Â Ô Ó ) L £ *
"4.AgG!
4>T69?8S6N UD9M)?M=@U @O9>?DM9S8[6S9QDR6MD?6#
¢èß
Qe
!
w~CGF1
gkJ=[U
r@
gD>S6XS6OS
Œ/
!
K
}WÙ°±:C8ßÓ

~
.B.AKHK"R6MO9=> $/($#
*""+
-/K}
--2
õ
‡×N1‚×ĜÓ
"%
"
#%
­ø
--2
×N1¶4‚×
ĜÓP
"%
+
#%
³ýH8
"E
"
#%
È÷H8
"E
+
#
˜
‡"
"-8D>>=>#
ÕÓ
"A#
‚opÕ—

"!
°±RL£
A?>@
üýþÿ!Ï<öÐq?
--2
«"#$
$#
þz…±‡˜
(
þzx[>?‡:¦1
--2
$?JòÃÁ±ãc1.†
!--2
×N1‚×ĜÓ
z
""JH%%%1&%
˜
F9""P#,#
Ë
&"JF#%?#%#!
¤Jû
+/,"
ø
"
¼
(#
­øîS­þ$?W_¡¢
$

"
ø
‚×Ĝ

÷67F
!
!×N1³ýH˜8
"E
"
#
û
#/#+, #/&%%!
¤J~û
#/+1&
!×N1È÷H˜8
"E
+
#
û
#/$,# #/*1$!
¤J~û
#/,#!
£‡"Õî¥
¨Ó
"A#
û
#/(%# "/#$(!
¤J~û
$/""
¨
$*
ø&
.îSFòòF]1
59Q6
op´ëßÓc§û
#/"1&
#/&1(!
¤Fˆ˜Q’x˜ˆ˜ô’xF]1
59Q6
op´ëßÓÁ”
!
û
#/&1(&
£Rcx˜‘’x
F]1
59Q6
op´ëßÓÁ»
!
û
#/"1&"
Ó)^Ž
±
#
A?A@
üýþÿ!Ï<öÐq?
Gi:.
ËT#$
z6
$
½Ë¾±
!,
þ
Gi:.
$?&J½ÃÁ±
ùä½Mô1.†
!
½Ëxy1.†;R;¡L¨
$###VU
ËD
!
Û>ÃÁ±
(,%
ø.†
"
¼
%#!
­þ$
?&åË1.†Óc§û
(" $#(
.
!
¤J­þ$
?ÃÁ
,#/,1
.
!
¤FÕu¥.†
",1
.
!
Õu¥µR
+&/(*0 *+/1$0!
¤JÕu¥µRû
1(/(+0!,
þ
$?þ
$*
øxS*1®LÀÐ
$##0
¤F$?&

K)GGJdg)JGA
1Õu¥Á”
" *+/1$0#!
£
K)
GAAdg)JCC
&1Õu¥Á»
"+&/(*0#
S*]
1
59Q6
op´ëßÓc§¨
#/,+("
Rcx˜‘’x
+%"
!!
"
!
#
!
$
"%
Ü
"
F]
# #/&+( "
ˆ˜Q’x˜ˆ˜ô’xF
]
#
6
$!
žsE{©®©àxS*¶ÙÚ1««¥
ÃÁ¡?õÑ6
"
$?&
K)GJGdg)JGG#
i9?Z$!]D>N9>?UDS6M>O=PU6DMQ@XS9RDMOD>N SWU6O
PM=[36>X9>?DM6D9> E86Y9D>?.M=R9>Q6VWGi:.
[DM\6MO@O9>?UM9[6MQ=[V9>DS9=> "K)GJGdg)JGG#
®¯G(à¼
$"$ $##
:D>6=PH=/M6P6M6N S=S86Q@XS9RDMO=MSWU6O!
T89Q8 T6M6X9OS6N 9> CDVX6$ "$ $##
J
B@
üýÿ!Ï<öÐq?
--2
«"#$
CDVX6(!C86Q8DMDQS6M97DS9=> =P--2X=Q9=PU6DM
DQQ6OO9=>OPM=[-=@S86M> DM6D=PE86Y9D>?.M=R9>Q6
$?
UM9[6M
‚×Ä
œÓ
%
"
¶4‚×
ĜÓ
%
+
³ý
H˜8
E
"
È÷
H˜8
E
+
‡"Õî
¥¨Ó
A
JF#(?$" 1 (/(1+ #/1"" #/1"% $/%&&
JF#%?#% & ,/+%, #/*(( #/*1$ "/#$(
F9"%P#% 1 "/### #/(*& #/+$% $/$%$
JH%%%1&% " $/($% #/$,1 #/"%, #/%#(
F9"(?$" ( $/**& #/+## #/%*% #/*",
F9#$Q$$ ( $/$*+ #/#+, #/$,# #/(%#
F9#&P#$ 1 %/*## #/1"" #/*$% $/1(+
F9""P#, " $/($% #/$,1 #/"%, #/%#(
JF#$F$# * %/#+# #/&%% #/11+ $/,++
#"V$ % (/#%" #/,,1 #/,&$ $/"(#
]AC"(V 1 "/&1( #/,,1 #/,*( $/(%*
HF##1V + (/+%$ #/11* #/1(* $/%$1
HF#$$V * +/($" #/11* #/*(+ $/*"1
¤J
[6D> +/," (/$* #/+1 #/,# $/""
J
C@
üýÏ<öÐq?
Gi:.
«"#$
CDVX6%!C86Q8DMDQS6M97DS9=> =PGi:.X=Q9=PU6DM
DQQ6OO9=>OPM=[E86Y9D>?.M=R9>Q6
$?&
UM9[6M
Q=[V9>DS9=>
ÃÁ†Ó
H=/=P
VD>N
Õu¥†
H=/=P
U=XW[=MU89Q
VD>N
ÕuÀ
U6MQ6>SD?6=P
U=XW[=MU89QVD>N
"0#
K)GGJdg)JGA %& %" *+/1$
K)GAAdg)JGG +# (, 1"/##
K)GAAdg)JCC (" $& +&/(*
K)GJAdg)JCJ %& (+ 1$/%(
K)GJGdg)JGG $#( 1* 1+/1(
K)GGJdg)JCG *$ +1 1#/1(
ÛK
S=SDX (,% ",1 ...
¤J
[6D> ,#/,1 %%/+# 1(/(+
6
"!
Ä0
--2
L£¢è1ž9¡©àxßQ`ße
i9?Z"!56>NM=?MD[=PU6DMDQQ6OO9=>OPM=[-=@S86M>
DM6D=PE86Y9D>?.M=R9>Q6VDO6N => --2D>DXWO9O
A?B@
üýþÿ!Ï<öÐq?˜Y€
û×6·&.îS]1_ê`ß
!
]~
4.AgG
à*¢èßQe
"
6
"!
6
(#
Ä0
--2
¢è1ž
9¡©àxßQ`ßeF
"
6
"#!
¨
-g
ßÓ
#/1#
d
„ßQeLû
%
ø;&
!
+
;
;&JKE{©®1î
S&è
!
¥ÙRcx
%
‹Gcx
%
¥x˜Yx

+
;
;&|K̹E{©®˜fQ©®1îS‹&è
!
IY½Lû
"
øe&
!
+We&¥Ù‘’x
(%
s1
’
%
x˜‘©

¤Fx˜‘©_ê`ß
a7
!
op´ë8û
#/&1"!
I—W¨W%Иs1’
WTèL›

+re&¨
-g
ßÓû
#/1*
dYL
û
"
R&
!
+
$
R&¥Ù•‡x
%
ˆ˜Q’x
%
ˆ˜
ô’x
%
ˆ˜x
%
,Žx
!
¤Fˆ˜Q’x˜ˆ˜
ô’x1´ë8û
#/&*,!
I—˜ˆ˜xTèWR

!
º¶ã”1´ë8
!
£•‡x˜æRL›W¨W
%
!
,ŽxטæR&1¤·îS1op´ë8ÁR

,%"
!"
È žs9¡©àxS*1
--2
˜
Gi:.
opÕî¥67
+
"
R&¥Ù̹E{1Qcx˜Œx

+
;;
;&
Jû̹fQ©®1‘’x
$
˜“”x
!
+
;h
;&ß
¶fQ1‘’x
"
6
(!
Ä0
Gi:.
¢è1ž9¡©àxßQ`ße
i9?Z(!56>NM=?MD[=PU6DMDQQ6OO9=>OPM=[-=@S86M>
E86Y9D>?.M=R9>Q6VDO6N => Gi:.[DM\6MO
!!
Ä0
Gi:.
¢è1ž9¡©àxßQ`ße
F
!
¨
-g
ßÓ
#/1,
d
!
ßQeLè
(
ø;&
"
6
(#!
+
;
;&YK
"
øe&&è
!
+We&¶
,
øîS&
è
$
ˆ˜x
%
ˆ˜Q’x
%
ˆ˜ô’x
%
•‡x
%
‘
’x
$
˜“”x
!
¤Fˆ˜Q’xRˆ˜ô’x
Ëã”1op´ë8
"#/&+(#
W¨W%
!
Rˆ˜xd
WèWøL›

+We&F눘x]¹E{©®
ð
!
¤·îSJ̹fQ©®
&
+re&JKE{©®
îS&è
!
¤Fx˜‘©Ë
#/&#%
1´ëßÓ
W¨W%Ð
!
dR,Žx
%
Yx
%
‹Gcx
%
¥x
%
Œ
x˜QcxW

+
;
;&¥ÙfQ1‘’x
"%
‘
’x
(%
s1’
!
¤F‘’x
(
˜s1’1op´ë8
û
#/*("
+
;;
;&߶̹E{1îSRcx

]~
H<-C-
-/F1
gkJ=[U
þWÙ°±:
C8r@×L£
!
Ä0
--2
Ó)¡èßQe1
gD>S6X
S6OS
Œv:ßÓû
&,/&&0!
£
Gi:.
1:ßÓû
&+/�
(!
ùú
7ÊÌ
!--2
˜
Gi:.
L…—xˤÛc1Mô
¥˜½ë¥‚»N
*"!(+
£ýHÿ~0x[>?1S*
© 
%
_ê`ߘopÕ67
*% $#+

÷67]
~
--2
˜
Gi:."
*—xþ7ÊÌzž9¡E{˜
fQ©®Œ¿1©àxS*r@ש ˜opÕî¥


z…±‡:¦1
$#
þ˜zx‡:¦1
(
þ
--2
$?F
!
Ë
JF#%?#%
1Õu¥Á”
!
© S*1
4ÀÁ”
$#
þ…±
--2
$?¨x‡ÃÁ±
,"
..
†
!

--2
$?¨7ê?*]1‰~¥
*$$+

÷67
F
!--2
×N1¤J‚×ĜÓû
+/,"!
»0þk1
67°±
*%!+!1!&!"(+
&
¤J³ýH8
#/+1!
º»0
a9[@MD
‚
*"(+
˜
]D=
‚
*++
1
#/,( Gi:.
Õu¥¨—
º±½×Ùë1°±
,
þ
Gi:.
$?¨ž9¡x
ìjF1¤JÕuÀû
1(/(+0!
»0~F¡xì
j1
*,0!
œž©àS*1
*10
*"%+
!
Iex[ìj
1
*&0
*,+
!
ç‰Í|œû÷67~®¯Ä÷û€
x
!
wW]¿©´þ¿F£ opÕþ/»

Ä0
--2
˜
Gi:."
ŸÓ)LêK}±1S*]
Á”
59Q6
op´ëßÓJ¨ˆ˜Q’xRˆ˜ô
’x]
"--2$ #/&1(&Gi:.$ #/&+(#!
£Á»
59Q6
o
p´ëßÓJ¨Rcx˜‘’x
"
]
" --2$ #/"1&&
Gi:.$ #/,+(#
¼·×
--2
˜
Gi:.
°±1WU¥

"
ŸÓ)å±1S*]Á”
59Q6
op´ëßÓ´
7
!
âÁ»
59Q6
op´ëßÓ~¦Xã;
!]D=
‚
*+!,+
Lê]~
--2
˜
Gi:.
—xþIexr@opÕî¥
L£3å±1opßÓc§º¶Ùëz{
!
½ò|
K
--2
˜
Gi:.
—x!¹õ‡1‹N<è1

g)
--2
˜
Gi:."
ŸÓ)Lê¡21ßQe°
±Ä÷WU
"
6
"
˜6
(#!
‡3
gD>S6XS6OS
Œv
"
ø
ßQe1:ßÓ
"&,/&&0
˜
&+/�#
º¼·×¤
½ë¥

ˆ˜’x|ž9¡fQ©®ˆ˜À1pQ
Ý¡
!
X8Åðζ
(##
ÕÊ1ƒ° ¡

¨žs
9¡1E{©®º¶íû
(
ˆ˜x
)
1S*

uÈË
Ì
!
þ0ˆ˜’x|WøS*ñ|WÙS*WœÇ¶


÷67F
!
ˆ˜x
%
ˆ˜Q’xRˆ˜ô’

"
øßQeF2W¨×W%
!
¸b×ã”1op
´ë8
!
â!¹2¶‹§.†
!
·¸[0õ‡1S*

ˆ˜xàá]¹E{©®
!
âa¸á|zfQ1ˆ˜
$*1

•‡x_¡ˆ˜À
!
¤c
%
’c
%

%
á’
J㈘’xp
*$+
!
¨
"
øßQeF
!
I˜ˆ˜x
%
ˆ
˜Q’x
%
ˆ˜ô’x2W¨W%
!
¸b×I—F
]ã71_ê`ß


!
̹E{1x˜‘©

!
̹fQ1‘’x
(
˜s1’
!
̹E{1Yx
%
¥x˜‹Gcx¨
"
øßQeFJLêW¨W%
!
¸b×·¸1©Ä¥

÷67F
!

(
ø‡íû‘’
x1S*
!
ýÜÝû‘’x
$%
‘’x
"
˜‘’x
(!
¨
"
øßQeFd1×¦ê
!
â2¸b×ç|
(
ø·¸õ‡1ĜÚ
!
5æ|‡Ý§?

Yx¨ž
s1;¡Lx®2¶L‡
!
g)Tu#`¢I½ò|
€x˜§x1H™*
*$+
!
¨Tu#‡R¤·xS*Ç
1%"
!"#$%&"()#*+&$,-$.*#/#$&0*.+%*+1
"#$#!"%""#$"%( "%*
¨ã;1¦§
!
÷67FI˜€xS*W¨W%
!
ï·
פ_÷FW½òû€x1`¢

àá
--2
˜
Gi:.
1ßQe°±Ä÷´A
!
â
¡L°±ñ¸b±×õWU

Ä0
Gi:.
Ó)¡2
1ßQeF
!
ëRcx¨4øßQe1g¡Fð
!
¤Ñ
]¹E{1îS2W¨W%
!
]¹fQ1îS×L‡
¨
"
ø;&F

£¨
--2
—x¡21ßQeF
!
Ì
¹E{˜fQ1îS¨+
"
;&F™¢W
!
w´¶
·¸®L:

ç½ò|K0
--2
˜
Gi:.
—xÃÁ
1Ĝ&¡×õ‡<è1¦§

÷67F
Gi:.
1
WÙ°±RS*1©ÄL‡z{=ÂA

%!
°k
÷67}~
--2
˜
Gi:."
*—xþ̹žE
{˜fQ©®1
$*
ø©àxS*¶ÙÚr@ש 
˜opÕî¥L£
!
íî·É×çØ©àxS*é¶
ãíÙ1opÕî¥

̹ˆ˜1
(
’x
)
R†¶ˆ
˜Ý1©àS*|é¶W _ê`ß1WÙS*
!

£1
(
ˆ˜’x
)
wm‚WS*
&
£‡íû
(
‘’x
)
1
(
øS*%ûõ‡1ĜÚ

çØ°±„þžs©
àxS*1rW^ǘÃ}~é¶MÍ1¨
-n~

jk!l
$
* $ +!
žs":Y#‰‹Ý67
Z
žsx1©àS*™š
*g+Z
Y#67쯫
!
±eL¤
! $&*#$$+ c("
* " +!.=T6X3! gDQ8MDWA! .M=RD> IZ.=XW[=MU89O[M6R6DX6N VWO9[UX6
O6L@6>Q6M6U6DSO*I+ZCM6>NO9> .XD>S-Q96>Q6! $&&,! $$ "$+ c"""
* ( +!h=O.! F=?6MO2! ]X66\6Mg! 269YD>Og! hD> 5K! :66C! F=M>6O
g! iM9YS6MOG! .=SI! .6X6[D> I! a@9U6Mg! EDV6D@ gZGi:.$!D
>6TS6Q8>9L@6P=M5HGP9>?6MUM9>S9>?*I+ZH@QX69QGQ9NO26O6DMQ8!
$&&+! "($ %%#1 c%%$%
* % +!]M9>93! gDMOg! F=M[D7DI;ZA6>6S9QN9R6MO9SW9> X=QDXC@>9O9D>
U6DMO"2<$#1*"CC#%.1:Z# OS@N96N T9S8 --2[DM\6MO*I+Z-Q96>S9D
F=MS9Q@XS@MD6! "##*! $$+$ ((1 c(%$
* + +!]D=:! J86> a! E8D>?5! JD=?6S9Q
N9R6MO9SWD>N O9[9XDM9SW=PU6DMQ@XS9RDMO>DS9R6S=KDOSGO9DM6R6DX6N
VW--2"O9[UX6O6L@6>Q6M6U6DS# [DM\6MO*I+ZA6>6S9Q26O=@MQ6
D>N JM=U KR=X@S9=>! "##1! +%$ &+& c&1$
* , +!]D=:! J86> a! E8D>?5! :9j! C6>? DOO6OO[6>S=P?6>6S9Q
RDM9DV9X9SWD>N M6XDS9=>O89UOT9S89> DO9D> U6DMOVDO6N => Gi:.
"G[UX9P96N iMD?[6>S:6>?S8 .=XW[=MU89O[# [DM\6MO*I+Z-Q96>S9D
F=MS9Q@XS@MD6! "##*! $$,$ (1% c(*#
* 1 +!
cˆ(
!
l5ƒ
!
Lh¥
!
r¦A
!
@uÁ
!
}†ø
Z
~F¡
xìjop3§˜_ê`ß1
--2

*I+Z
±e#$
! "##1!
"%$ ",* c"1+
* * +!
l5ƒ
!
cˆ(
!
}†ø
!
@uÁ
!
V¦§
!
r¦A
Z
~F¡
xìj1
Gi:.

*I+Z
±e#$
!"##*! "+"(#$ "&* c(#%
* & +!3t>OQ8 G! F=M[D7DI;ZJ8DMDQS6M97DS9=> =PRDM9DV9X9SWD>N ?6>6S9Q
O9[9XDM9SW=PK@M=U6D> U6DM@O9>?[9QM=ODS6X9S6X=Q9N6R6X=U6N 9>
DUUX6*I+Z-Q96>S9DF=MS9Q@XS@MD6! "##1! $$($ (1 c%(
*$#+!9J! -DTD[@MD-D9S=C! a=S=V@\9a! FDWDO89CZ;>S6?MDS6N 26P6M6>Q6A6>6S9Q
X9>\D?6[DUO=PU6DMVDO6N => --2D>N Gi:.[DM\6MO*I+Z]M66N9>?
-Q96>Q6! "##1! +1$ ("$ c("&
*$$+! C! gDSO@SDHZ--2O9O=XDS6N PM=[DUUX6QD> 9N6>S9PWU=XW[=MU89O[
D>N ?6>6S9QN9R6MO9SW9> U6DM*I+ZC86=M6S9QDXD>N GUUX96N A6>6S9QO!
"##$! $#"$ *,+ c*1#
*$"+!C6>?<3! CD>DV6a! CD[@MDP! ;SD9GZA6>6S9QM6XDS9=>O89UO=P
U6DMQ@XS9RDMO9> j9>Y9D>?! J89>DDO[6DO@M6N VW2G.5[DM\6MO
*I+ZI=@M>DX=PF=MS9Q@XS@MDX-Q96>Q6D>N ]9=S6Q8>=X=?W! "##$! 1,$
11$ c11&
*$(+!DV6C! a=S=V@\9a!
FDWDO89C! ]D> Q6M6U6DSOP=M?6>6S9Q
D>DXWO9O9> U6DM*I+ZK@U8WS9QD! "##"! $"%$ $"& c$(1
*$%+! S=P[9QM=ODS6X9S6
[DM\6MO9> IDUD>6O6U6DM"2<$#13<$.(".& HD\D9# *I+Zg=X6Q@XDM
KQ=X=?WH=S6O! "##"V! "$ $% c$,
*$++!A9D>PMD>Q6OQ89:! -6?X9DOH! CDMQ89>92! a=[YD>Qg! A6OOX6MJZ
-9[UX6O6L@6>Q6M6U6DSOP=MS86?6>6S9QD>DXWO9O=PDUUX6*I+Z
C86=M6S9QDXD>N GUUX96N A6>6S9QO! $&&*! &,$ $#,& c$#1,
*$,+!A@9XP=MN .! .MD\DO8 -! E8@ Ig! 29\\6M9>\ K! ADMN9>6M-! ]DOO6S
F! i=MOS6M2Zg9QM=ODS6X9S6O9> gDX@Oj N=[6OS9QD"DUUX6#$
DV@>ND>Q6! U=XW[=MU89O[ D>N Q@XS9RDM 9N6>S9P9QDS9=> * I+Z
C86=M6S9QDXD>N GUUX96N A6>6S9QO! $&&1! &%$ "%& c"+%
*$1+!-9XPR6MV6M?)N9XT=MS8 K! gDSDOQ9J:! hD> 5K! 36?3 K! hD>
aDD@T6> g.3! 3DXO6Mg! a=NN6:.! -=?X9=h! A9D>PMD>Q6OQ89
:! 5@M6XJK! J=OSDi! .DS=QQ89GZg9QM=ODS6X9S6[DM\6MOOUD>>9>?S86DUUX6"RT
6"C+1/.*& ]=M\8Z# ?6>=[6*I+ZCM66A6>6S9QOrA6>=[6O! "##,!
"$ "#" c""%
*$*+!
M
!
I
!
ô
!
ä
!
}†ø
!
 ·8
!
4
!
¨
!
F--2

*I+Z
‹Ý#$
!"##1! (%"$#$ %1 c+"
*$&+!]MD>SI! JD6SD>=)D>=X6OA! AM6OO=P.gZiDOSD>N O6>O9S9R6O9XR6M
OSD9>9>? =P 5HG 9> U=XWDQMWXD[9N6 ?6XO* I+Z G>DXWS9QDX
]9=Q86[9OSMW! $&&$! $&+$ *# c*(
*"#+!2=8XPiIZH@[6M9QDXSDk=>=[WD>N [@XS9RDM9DS6D>DXWO9OOWOS6[Z
h6MO9=>"Z#"*g+ZH6T<=M\$Kk6S6M-=PSTDM6! $&&*
*"$+!H69g! :93ZgDS86[DS9QDX[=N6XP=MOS@NW9>??6>6S9QRDM9DS9=> 9>
S6M[O=PM6OSM9QS9=> 6>N=>@QX6DO6O*I+Z.M=Q66N9>?O=PS86HDS9=>DX
GQDN6[W=P-Q96>Q6=P4>9S6N -SDS6O=PG[6M9QD!$&1&!1,$ +",& c
+"1(
*""+ !<68 iJ! ?2JZ.B.AKHKhK2-;BH$Z($$ g9QM=O=PS
T9>N=T)VDO6N PM66TDM6 P=MU=U@XDS9=> ?6>6S9Q D>DXWO9O* g+Z
4>9R6MO9SW=PGXV6MSDD>N J6>SM6P=M;>S6M>DS9=>DXi=M6OSMW26O6DMQ8!
$&&&
*"(+!a9[@MDC! -89]D> S9P9QDS9=> =PGO9D> U6DMRDM96S96OVW--2
D>DXWO9O*I+Z]M66N9>?-Q96>Q6! "##"! +"$ $$+ c$"$
*"%+!g=>S6)Q=MR=:! JDVM9SD:! BX9R6M9MDJ! :69SD=IZGOO6OO[6>S=P
.WM@OQ@XS9RDMOVW2G.5D>N Gi:.[DM\6MO*I+ZA6>6S9Q26O=@MQ6O
D>N JM=U KR=X@S9=>! "###! %1$ "+1 c",+
"
€‚
!
rƒƒ
#
*%"