---

作 者 ：李蕾,彭存智,李明芳,王向社,郑学勤

期 刊 ：热带亚热带植物学报 2008年 16卷 6期 页码：537~544

关键词：荔枝；胚发育；蛋白质图谱；质谱；

Keywords:Litchi, Embryo Development, Proteomics mapping, MALDI-TOF-MASS,

摘 要 ：通过二维聚丙烯酰胺凝胶电泳以及计算机辅助的图像分析技术, 对荔枝开花后40 d的正常与败育胚蛋白质图谱进行初步分析。结果表明, 100个蛋白质点在表达丰度上有明显差异; 选择仅在正常发育胚胎胶上表达的蛋白点15个和仅在败育胚胎胶上表达的蛋白点50个, 进行基质辅助激光解吸附电离飞行时间质谱(MALDI-TOF-MASS)分析, 鉴定出9个与胚发育相关的蛋白, 这些蛋白可能参与了胚败育的调节和控制。

Abstract:The global protein patterns in normal and aborted embryo of litchi at 40 days after flowering (DAA) were compared by using two-dimensional polyacrylamide gel electrophoresis (2-DE) and computer image analysis. The results showed that the quantities of 100 protein spots in normal embryo were significantly different from aborted embryo. There were 15 and 50 spots appeared on the gel of normal embryo and aborted embryo, respectively. Then they were selected for MALDI-TOF-MASS analysis. Nine proteins related with embryo development were identified, which maight involve in regulating and controlling the embryo abortion.

---