Abstract:Semi-quantitative RT-PCR was performed to detect the expression pattern of phenylalanine ammonia-lyase (PAL) gene during Phalaenopsis sp. leaf explant browning. Transcript RNA of PAL was rapidly increased at day 3 after in vitro culture, maintained at a high level until day 8 after culture and then declined. PAL mRNA was present one day earlier than the control after cultured on MS containing 55.6mg/L FeSO4, and PAL activity was higher than the control. The total phenol content in leaf explants on both media increased with browning. It is suggested that PAL gene expression is associated with Phalaenopsis sp. leaf explant browning.