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作 者 ：葛丽娜;韩雪;任珂珂;章甫;彭永宇;毕淑峰*

期 刊 ：植物研究 2014年 34卷 2期 页码：276-281

关键词：火棘花；挥发油；化学成分；GC-MS；抗氧化活性；

Keywords:Pyracantha fortuneana flowers, volatile oil, chemical constituents, GC-MS, antioxidant activity,

摘 要 ：采用水蒸气蒸馏法提取火棘花挥发油，利用气相色谱—质谱联用技术（GC-MS）分析其化学成分。使用维生素C和BHT为阳性对照，以DPPH自由基、亚硝酸钠清除作用为指标评价挥发油的抗氧化活性。从挥发油中鉴定了77个化合物，占挥发油总量的83.77%，含有多种生物活性成分，以萜类及其含氧衍生物（50.31%）、烷烃（18.52%）、醛（5.54%）为主；挥发油对DPPH自由基、亚硝酸钠有明显的清除作用，清除率为50%时，其体积分别为43.51、79.48 μL，样品量与清除率间呈量效关系；挥发油对DPPH自由基的清除效果略低于1 mg·mL^-1维生素C，对亚硝酸钠的清除效果优于1 mg·mL^-1的BHT。

Abstract:The volatile oil from Pyracantha fortuneana flowers was extracted by watersteam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·and sodium nitrite scavenging capacity with VC and BHT as the positive controls. Seventy-seven compounds, which occupied 83.77% of total constituents and contained many bioactive constituents, were identified. Terpenes and their derivatives (50.31%), alkanes (18.52%), and aldehydes (5.54%) were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·and sodium nitrite in a concentration-dependent fashion. When the scavenging rate against DPPH· and sodium nitrite of volatile oil was 50%, the volumes of volatile oil were 43.51 and 79.48 μL, respectively. The scavenging capacity against DPPH· of volatile oil was slightly worse than that of 1 mg·mL^-1 Vc and the scavenging capacity against sodium nitrite of volatile oil was better than that of 1 mg·mL^-1 BHT.

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