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期 刊 ：植物研究 2015年 35卷 3期 页码：476-480

关键词：塔拉纤维剩余物；纤维素酶；酶解；还原糖产率；

Keywords:Tara fiber residues, cellulase, enzymatic hydrolysis, yield of reducing sugar,

摘 要 ：富含单宁的塔拉豆荚经水解制备没食子酸，其剩余物中富含纤维素。本研究探讨了酶解各因素对塔拉纤维剩余物还原糖产率的影响，在单因素实验的基础上，进行料液比、酶解温度、pH和酶解时间四因素L₁₆(4)⁵正交优化实验，其优化工艺条件为料液比1∶6 g·mL^-1，酶解温度55℃，pH6，酶解时间48 h。在此条件下，酶解塔拉纤维剩余物还原糖产率均值为43.95%。

Abstract:Tannin from Tara pods is hydrolyzed for preparation of gallic acid, whose residues is rich in cellulose. We discussed the effects of various factors on the yield of reducing sugar from Tara fiber residues. By the single factor experiment, we optimized four factors including the ratio of solid-liquid, enzymatic temperature, pH and enzymatic time by orthogonal experiments L₁₆(4)⁵. The optimum conditions were under the ratio of solid-liquid of 1∶6 g·mL^-1, enzymatic temperature of 55℃, pH of 6 and enzymatic time of 48 h, and the yield of reducing sugar from Tara fiber residues was 43.95%.

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