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作 者 ：徐斌;朱报著;张方秋;潘文;王裕霞

期 刊 ：植物研究 2014年 34卷 4期 页码：479-484

关键词：红花荷；ISSR；遗传多样性；

Keywords:Rhodoleia championii, ISSR, genetic diversity, genetic structure,

摘 要 ：利用ISSR分子标记技术，对红花荷主要分布区8个天然群体的遗传多样性和遗传结构进行研究。结果表明：红花荷在物种水平上其多态位点百分率（PPB）为94.86%,Shannon表型多样性指数（H₀）为0.343 8;在群体水平上PPB为73.07%，H₀为0.276 3，说明红花荷群体具有丰富的遗传多样性。AMOVA分子方差分析表明,红花荷遗传变异主要来自群体内，群体内遗传变异占群体的70.29%,群体间占29.71%。Mantel检测表明红花荷群体间的遗传距离与地理距离之间没有明显相关性。

Abstract:The genetic diversities and genetic structures of 8 Rhodoleia championii wild populations were evaluated using ISSR markers. The results showed that R.championii had higher genetic diversity at both species level (percentage of polymorphic bands (PPB)=94.86%, Shannon’s phenotypic diversity index (H₀)=0.343 8) and population level (PPB=73.07%, H₀=0.267 3). The analysis of molecular variance (AMOVA) showed that genetic differentiation among and within populations was all found to be remarkably high, and 29.71% of genetic variation existed among populations. The Mantel test showed that the geographic and genetic distances were not significantly correlated to each other.

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