Abstract:An investigation on the bryophyte species diversity in main forest vegetations was made in upper valley reach of Chishui river, Guizhou province, China. The results showed: (1)There were 28 taxa of bryophytes belonging to 19 genera and 15 families and the life-forms are wefts (64.29%), short turfs (28.57%) and mats (7.14%). (2)The species richness of bryophytes was the largest in bamboo forest (2.131), remnants of broadleaved forest was second (0.687) and the smallest in coniferous forest (-1.444). (3)α diversity index of bryophytes is bamboo forest (3.170)>remnants of broadleaved forest (3.000)>artificial vegetation area (2.807)>shrubbery forest (2.322)>coniferous forest and top valley area (1.585). (4)β diversity index of bryophytes was the highest between shrubbery forest and remnants of broadleaved forest (1.000), the second is between Bamboo Forest and Remnants of Broadleaved Forest (0.938) and the smallest is between shrubbery forest and coniferous forest (0.857). The biodiversity of bryophytes was correlated with forest vegetation, shade degrees and humidity in the forest. Research on the biodiversity of bryophytes is good for protection of biodiversity and providing scientific data and information.