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作 者 ：路祺；高悦；项凤影；祖元刚*；张莹

期 刊 ：植物研究 2015年 35卷 1期 页码：154-157

关键词：牡丹种荚；水浴；多糖；正交实验；

Keywords:peony seed pods, water bath, polysaccharide, orthogonal test,

摘 要 ：以牡丹种荚作为原料，水为提取溶剂，先采用单因素实验方法探讨提取温度（40~100℃）、提取时间（15~240 min）和料液比（1∶5~1∶40 g·mL^-1）对牡丹种荚多糖提取率的影响，并通过正交实验优化工艺条件，确定最优提取工艺为：提取温度100℃，提取时间60 min，料液比1∶15 g·mL^-1。此条件下，牡丹种荚多糖提取率为8.34%。水提牡丹种荚多糖，方法简单，无环境污染和溶剂回收问题，本文为牡丹种荚综合开发利用提供实验依据。

Abstract:We investigated polysaccharide extraction yield from peony seed pods by single-factor experiments with water extraction method under extraction temperature of 40-100℃, extraction time of 15-240 min and solidliquid ratio of 1∶5-1∶40 g·mL^-1. Under the optimum extraction process of orthogonal test, extraction temperature of 100℃, extraction time of 60 min and solidliquid ratio of 1∶15 g·mL^-1, the polysaccharide extraction yield reached to 8.34%. Water extraction method has the advantages of the simple process, no environmental pollution and no solvent recovery problem. Our results can provide the experimental evidences for peony seed pod utilization.

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