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作 者 ：马春泉;张　莹;崔　颖;王　冰;王玉婷;刘金玲;王宏建;李海英*

期 刊 ：植物研究 2008年 28卷 4期 页码：408-411

关键词：cDNA文库；EST；表达载体；噬菌斑原位杂交；

Keywords:cDNA library, EST, expression vector, plaques in situ hybridization, method of RACE,

摘 要 ：构建了甜菜M14品系在花期的ZAP表达载体的cDNA文库，利用抑制消减杂交方法所获得的M14品系特异表达的2个EST片段为探针筛选cDNA文库，获得了M14品系特异表达cDNA片段Me-86和Me-84；进一步利用RACE技术获得了基因M14-86 cDNA片段的全长序列。生物信息学分析表明M14品系特异表达基因M14-86的cDNA片段与cDNA片段Me-84分别与大花马齿苋(Portulaca grandiflora)及瓶子草(Sarracenia purpurea)的26S核糖体RNA具有很高的同源性。

Abstract:The cDNA library of sugar beet M14 in florescence was constructed using ZAP expression vector. Two ESTs, which were specifically expressed in M14 and obtained by suppression subtractive hybridization, were used as probes to screen the cDNA library. Then, two cDNA fragments Me-84 and Me-86 specifically expressed in M14 were obtained. Full length cDNA sequence of gene M14-86 was later obtained using the method of RACE. Bioinformatic analysis of M14-86 and Me-84 cDNA fragments showed high homology with the 26S ribosomal RNA gene in Sarracenia purpurea and Portulaca grandiflora, respectively.

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