Abstract:The effects of GA3 and IAA with different concentrations and combinations on the dynamic accumulation of holocellulose and chlorophyl content in Neosinocalamus affinis were studied. The objective of the study is to provide theoretic basis for genetic improment of bamboos used in pulp industries. The results showed that the effects of GA3 and IAA with different concentrations and combinations on the dynamic accumulation of holocellulose content were different. In 30 days after the treatments, the accumulation intensity of holocellulose was much higher under the GA350IAA200 treatment than under the GA3200IAA50 treatment. The result was inverse after 30 days (40 to 50 days). The final results revealed that the content of holocellulose was significantly higher at 0.01 level under the GA3 and IAA treatments than under the control treatments. GA3 and IAA were generally capable of improving the accumulation of chlorophyl content. The chlorophyl content in GA3200IAA50 was higher than that of GA350IAA20 treatments. The results of correlation analysis showed that the relationships of chlorophyl content with holocellulose content were not significant and positive.