Abstract:In order to creat wheat-rye translocations which have very important value in wheat breeding and production, we selected the lines with common wheat rye’s characters in the crossing between wheat-rye substitution line 5R/5A and 6R/6A. Now we have obtained 9 lines of F6, 6-26，06-60-11，06-6-24，06-6-15，06-6-35，6-34，6-28，06-6-17，06-6-14. These lines are useful for the genetic stability and fertility and dry and disease resistance and they have multispikelets with more number of grains per ear, higher grain weight, more ears per plant as well as other good characters. Twenty primer pairs located on chromosomes 5R and 6R were used for SSR amplification in these lines and their parents.The results showed that the molecular marker SCM268 was singled out from 20 primers，which could amplify the specific molecular marker SCM268150 of rye in 8 of 9 lines,6-26,06-60-11，06-6-15，06-6-35，6-34，6-28，06-6-17 and 06-6-14.It can be deduced from the result that the 8 lines are wheat-rye translocation lines which have very important value in production and wheat breeding.