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作 者 ：季必霞;陈达伟;张晨晨;闵笛;黄文静;王钰;*

期 刊 ：植物研究 2011年 31卷 5期 页码：558-562

关键词：大花蕙兰；秋水仙素；化学诱变；

Keywords:Cymbidium hybridium, colchicine, chemical induction, polyploid,

摘 要 ：以大花蕙兰‘红瀑布’无菌苗丛芽为材料、秋水仙素为诱变剂，采用不同的处理浓度、时间诱导大花蕙兰体细胞加倍。通过形态学和细胞学观察、统计等方法对其进行倍性鉴定。结果表明：秋水仙素浓度0.05%，处理时间24 h的条件下，诱导率高达28.2%；多倍体苗外部形态、叶绿体数目、气孔数目和大小与二倍体差异大，加倍后的细胞核明显变大，染色体倍数增加。

Abstract:The shoots of Cymbidium hybridium were soaked in colchicine solutions at different concentrations and treatment times to induce doubling of body cell chromosome. Ploidy levels were identified by morphological and cytological traits. The results showed that the treated shoots of C.hybridium with 0.05% colchicine for 24 h had a higher inducing rate which was up to 28.2%. The external morphology, the number of chloroplast as well as the number and size of stomata were obviously different between the diploid and polyploid. The nucleus of polyploid cell was obviously bigger and the number of chromosome was increased.

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