Abstract:The cytogenetics of the cross-progenies between Chinese Spring-Agilops 2C disomic addition and Chinese Spring-Elytriga disomic addition were studied. The results showed that The highest seed-setting of crossing generation is 36.06%, the lowest is 19.43% and average is 29.5%. The highest seed-setting of F1-selfed is 62.09%, the lowest is 30.92% and average is 39.64%. the control experiment and analysis of variance showed that the gametocidal chromosome effected on the difference of seed-set rate of hybridization in different lines. After scrutinizing the meiosis of pollen mother cells (PMS) in F1 hybrids, the results were concluded: the number of univalents exceeded the expected and some trivalents and tetravalents appeared at the same time. These were mainly caused by the gametocidal chromosome 2C.