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作 者 ：洪森荣;尹明华*

期 刊 ：植物研究 2010年 30卷 2期 页码：191-196

关键词：香果树；种质；离体保存；

Keywords:Emmenopterys henryi Oliv., germplasm, conservation in vitro,

摘 要 ：以香果树（Emmenopterys henryi Oliv.）带芽茎段为外植体，以MS为基本培养基，采用正交实验和单因子实验方法研究了培养温度、无机盐水平、蔗糖、甘露醇和植物生长抑制剂PP₃₃₃对香果树试管苗离体保存的影响。结果表明，香果树带芽茎段在9℃低温下保存于附加2.0 mg·L^-1 KT、2.0 mg·L^-1 6-BA、0.1 mg·L^-1 NAA、50 g·L^-1蔗糖和10 g·L^-1甘露醇的1/2MS培养基上，180 d后其成活率可达80%。PP333对香果树的离体保存也有显著影响，其中最适合的PP₃₃₃浓度为6 mg·L^-1，180 d后其成活率可达95%以上。离体保存后的试管苗经形态指标和生理生化指标测定以及RAPD分析检测没有发生遗传变异。本实验结果为珍稀濒危植物香果树的种质保存提供了一条简单有效的途径。

Abstract:The effects of culture temperature, inorganic salts level, sucrose, mannitol and plant growth inhibitor PP₃₃₃ on in vitro conservation of Emmenopterys henryi Oliv. stems with a bud in MS basic medium were studied using singlefactor experiments and orthogonal experimental method. The results showed that the survival rate of E.henryi Oliv. stems with a bud cultured in 1/2MS medium containing 2.0 mg·L^-1 KT, 2.0 mg·L^-1 6-BA, 0.1 mg·L^-1 NAA, 50 g·L^-1 sucrose and 10 g·L^-1 mannitol and stored at 9℃ low temperature was up to 80%. PP333 also had a significant effect on in vitro conservation of E.henryi Oliv., the most suitable concentration of PP₃₃₃ was 6 mg·L^-1, the survival rate after 180 d was over 95%. After morphological indexes measuring, physiological and biochemical indexes determination and RAPD analysis detection, the genetic variation of E.henryi Oliv. conservation in vitro plantlets did not appear. The results provided a simple and effective way for in vitro conservation of the rare and endangered plants E.henryi Oliv. germplasm.

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