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作 者 ：王吉凤;李青;包欣

期 刊 ：植物研究 2012年 32卷 1期 页码：84-90

关键词：芍药；外植体；组织培养；污染；抗菌素；

Keywords:peony, explant, tissue culture, contamination, antibiotics,

摘 要 ：针对芍药在组织培养过程中外植体污染难于控制的问题进行了研究。试验分别于不同的季节取材后对外植体进行不同的表面消毒处理。结果表明：以芍药带芽茎段、顶芽、侧芽为外植体，采用0.1%~0.2% HgCl2消毒8 min，结合母株黄化处理、4℃消毒液+搅拌的表面消毒处理，可降低外植体初期培养污染率；在培养基中添加抗菌素，可明显控制内生菌的污染。

Abstract:To solve the problem of explant contamination during peony tissue culture, the explants of different seasons were studied. The results showed that soaking explants in 0.1%-0.2% HgCl2 for 8 min, etiolation treatment, low temperature and electronic vibration are effective for sterilization; Antibiotics added into the media can inhibit contamination caused by endophytes.

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