Abstract:The differences of fluorescence parameters of hermaphrodite gametophyte and male gametophyte from Ceratopteris thalictroides cultured in MS medium and modified knop’s medium were investigated. The results showed that the ФPSⅡ and ETRPSⅡ of hermaphrodite gametophyte in MS medium were higher than those of the male gametophyte in MS medium and gametophytes in modified knop’s medium, the differences were significant. The values of Fv/Fm had no significant difference in the gametophytes of different groups. The external nutrient had no obvious effect on the qP and qN in the gametophytes of different teams. In conclusion, external sucrose enhanced photosynthetic activity of the gametophytes in MS medium, which was beneficial for hermaphrodite gametophytes to grow and develop rapidly.