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作 者 ：鲁巍巍;吕德国;*;李志霞;周传生;

期 刊 ：植物研究 2009年 29卷 2期 页码：248-252

关键词：绿肉山楂；叶片；黄酮；反相高效液相色谱法；

Keywords:Crataegus chlorosarca Maxim., leaves, flavonoids, RP-HPLC,

摘 要 ：为充分利用山楂叶用资源，采用反相高效液相色谱法，以ZORBAX Extend-C18色谱柱（250×4.6 mm，5 μm），乙腈-0.5%磷酸水溶液梯度洗脱，流速1.0 mL·min^-1,检测波长345 nm，柱温25℃，对不同生长时期的绿肉山楂叶中牡荆苷、牡荆素2″-O-鼠李糖苷、金丝桃苷、芦丁和槲皮素含量进行测定。结果表明，在此分析检测条件下牡荆苷、牡荆素2″-O-鼠李糖苷、金丝桃苷、芦丁和槲皮素的精密度、重复性、稳定性及平均加样回收率试验的RSD值均小于3%，方法准确可靠。绿肉山楂叶中含有的黄酮类成分主要以牡荆苷和金丝桃苷为主，且二者含量变化规律与其总黄酮含量变化相似，均在10月初含量达到最高；此时采摘，其质量完全符合《中国药典》的有关规定，故绿肉山楂可作为一种新的药用资源来进一步开发利用。

Abstract:To fully use Crataegus leaves resources, experiment was conducted with C. chlorosarca Maxim. leaves as materials. The HPLC conditions with ZORBAX Extend-C18 column（250×4.6 mm，5 μm）was used, the mobile phase consisted of acetonitrile -0.5% phosphoric acid with gradient elution at flow rate of 1.0 mL·min^-1, the detection wavelength was set at 345 nm and the column temperature was 25℃ to develop a HPLC method for the quantitative analysis of vitexin, rhamnosylvitexin, hyperoside, rutin and quercetin.The results showed that the RSD of precision,reproducibility, stability and recovery results of five flavonoids was not exceed 3%. The methods is accurate and reliable. The main flavonoids of C. chlorosarca Maxim. were vitexin and hyperoside, whose changes were similar to that of the total flavonoids, and the highest contents were in October. The quality of leaves from C. chlorosarca Maxim. picked in October consistents with China Phamacopeia, so C. chlorosarca Maxim. can be used as a new medical resource.

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