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作 者 ：张丽杰;张丽玮;冯丹丹;赵　霞;沈海龙*

期 刊 ：植物研究 2007年 27卷 3期 页码：319-324

关键词：水曲柳；离体快繁；腋芽；

Keywords:Fraxinus mandshurica, in vitro culture, axillary buds,

摘 要 ：以水曲柳带顶芽、腋芽茎段为外植体进行离体培养，研究其适宜的灭菌方法、基本培养基种类和激素对腋芽萌发、丛芽产生、芽苗增殖的影响。结果表明，水曲柳的腋芽茎段为快繁的适宜外植体，茎段灭菌以用0.05%HgCl₂处理2 min最好。在萌芽培养中，BA和2ip均可促进腋芽萌发，但以8 mg·L^-1 BA处理时萌发效果最好，萌发率达100％；将腋芽萌发后长成的新枝转入添加ZT的培养基中，出现丛芽，在添加1.0 mg·L^-1的ZT的培养基中增殖效果最好，增殖系数达到3.0。无论在萌芽培养还是增殖培养中均发现WPM培养基最适合水曲柳腋芽的离体快繁。

Abstract:The proper sterilizing method, the effects of basic media and hormones for axillary bud sprouting and proliferation in vitro were studied. The explants were stem cuts with axillary or apical bud from one-year old seedlings of Fraxinus mandshurica. The sterilization was proved to be very difficult. Sterilizing the buds with 0.05% HgCl₂ for 2 min resulted in the lowest contamination. The effect of BA for bud germination was better than that of 2ip. Eight mg/L was proved to be the optimum level of BA, and the bud sprouting rate could reach 100% by this level. Shoot cluster could be obtained by transplanting the sprouted axillary buds onto medium with ZT, the best proliferation was observed on medium with one mg/L ZT, and the proliferation rate could reach three. WPM was proved to be the best medium either for the bud sprouting and shoot proliferation, that is, WPM medium is proper for in vitro axillary bud culture of F. mandshurica.

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