Abstract:In order to explore the antitumor active components of Typhonium giganteum Engl, the powder of T.giganteum Engl tubers was extracted using supercritical fluid CO2-80% ethanol, and the extract was identified by gas chromatography-mass spectrometry(GC-MS) technology. The in vitro antitumor effects of the extract was evaluated by MTT colorimetric method. Thirty-seven compounds were identified from the extract by GC-MS analysis. Four major components are as follows: β-Sitosterol, accounting for 40.22%; Campesterol, accounting for 18.45%; n-Hexadecanoic acid, accounting for 9.52%; 9,12-Octadecadienoic acid (Z,Z)-, accounting for 8.15%. MTT experiments indicated that the extract showed significant inhibition on the colon cancer HCT-8, ovarian cancer HO-8910, gastric cancer SGC-7901, and liver cancer SMMC-7721 cells. Liver cancer SMMC-7721 cells were the most sensitive to the extract. The extract of T.giganteum Engl tubers by supercritical fluid CO2 showed remarkable anti-tumor effect. Further research on this extract is necessary to find its active substances for reasonable development and utilization of T.giganteum Engl.