---

作 者 ：李春英;赵春建;张宝友;刘帅华;刘德曼;祖元刚

期 刊 ：植物研究 2012年 32卷 (3)期 页码：373-376

关键词：烟草；辅酶Q10；匀浆提取；正交试验；

Keywords:tobacco, CoQ10, homogenate extraction, orthogonal experiment,

摘 要 ：以烟草叶片为原料，用正交试验法优化了烟草中辅酶Q₁₀的匀浆提取工艺，考察了匀浆时间、乙醇体积分数、液料比和提取次数4个因素对辅酶Q₁₀提取率的影响，确立了烟草中辅酶Q₁₀的优化匀浆提取工艺条件为:提取溶剂为85%乙醇，匀浆时间6 min，液料比9∶1(mL/g)，提取次数2次。将该法与索氏提取进行了对比。结果表明，匀浆提取烟草中辅酶Q₁₀的提取率为12.82 μg·g^-1，与索氏提取的提取率相当，但匀浆提取所用提取时间短，提取溶剂用量少，因此匀浆法具有明显的优势。

Abstract:Using tobacco leaves as materials, the homogenate extraction technology of coenzyme Q₁₀ (CoQ₁₀) in tobacco was optimized by orthogonal experiments. The effect of homogenate time, ethanol volume percent, ratio of liquid to material and number of extraction cycles on the extraction yield of CoQ₁₀ was investigated. The optimized homogenate extraction technology was determined as follows: 85% ethanol as extraction solvent, homogenate time 6 min，ratio of liquid to material 9∶1(mL· g^-1), 2 extraction cycles. A comparison between homogenate and Soxhlet extractions was conducted. The extraction yield of CoQ₁₀ was 12.82 μg·g^-1 by homogenate extraction, which was almost equivalent to Soxhlet extraction. Homogenate extraction is faster and needs less solvent, thereby homogenate extraction has an advantage over Soxhlet extraction.

---