Abstract:Based on the preliminary experiments, a three-variable, three-level Central Composite Design (CCD) was performed to study the ultrasound-assisted extraction of 2″-O-galloylhyperin from Pyrola calliantha H.Andr.. The optimum parameters were as follows: extraction time 45 min, ultrasound power 40 kHz, three extraction cycles, extraction temperature 60℃, liquid/solid ratio 33∶1, ethanol concentration 50%. Under the optimal conditions, the average extraction yields of 2″-O-galloylhyperin was 5.024 mg·g-1.