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期 刊 ：植物保护学报 2008年

关键词：二苯醚类除草剂；花生；氮素代谢；硝酸还原酶；谷氨酰胺合成酶；

Keywords:diphenylethers herbicides, peanut, nitrogen metabolism, NRase, GS,

摘 要 ：在温室条件下,研究三氟羧草醚和乙羧氟草醚对苗期花生生长发育以及氮素代谢的影响。结果表明:两种除草剂均明显抑制苗期花生的生长发育,同时抑制苗期花生的氮素代谢过程中关键酶硝酸还原酶和谷氨酰胺合成酶的活性,导致苗期花生植株的全氮含量显著降低。其中三氟羧草醚对花生生长发育的抑制作用较强,在高剂量下,对花生植株鲜重抑制率、干重抑制率分别为32.85％和30.00％;与对照花生的结瘤率(80.00％)相比,三氟羧草醚处理的花生结瘤率为28.60％;叶片叶绿素含量在处理后各时期均低于空白对照。三氟羧草醚和乙羧氟草醚对花生氮素代谢的关键酶硝酸还原酶和谷氨酰胺合成酶活性均有不同程度的抑制作用,影响了花生氮素的正常代谢,花生植株全氮含量分别为1.452％和1.553％,显著低于对照植株全氮含量。

Abstract:The effects of acifluorfene and fluoroglycofen-ethyl on growth and nitrogen metabolism of peanut at the seedling stage were studied in the greenhouse. Two herbicides had strong inhibitory effects on growth development of peanut and key enzymes activity, nitrate reductase and glutamine synthetase, leading to the plant total nitrogen content of peanut decreased observably. The effect of acifluorfene on growth of peanut was shown to exert a fairly strong inhibitory and inhibitory rate of fresh weight and dry weight were 32.85% and 30.00%, and nodulation rate was 28.60%, comparison with nodulation rate without herbicides 80.00%. Moreover, contents of chlorophyll in the treatment were lower than those of control. Acifluorfene and fluoroglycofen-ethyl had inhibitory effects on key enzymes activity, nitrate reductase and glutamine synthetase, during N metabolism to different extents. The total nitrogen contents of acifluorfene and fluoroglycofen-ethyl treatments were 1.452% and 1.553％ respectively, which were obviously lower than that of blank control group.

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