Abstract:In order to study the diversity of Meloidogyne incognita associated bacteria, and analyze the interaction between M.incognita and associated bacteria, a fosmid library of genome of M.incognita associated bacteria has been constructed by the ways of Nycodenz centrifuge, sodium dodecyl sulfate (SDS) lysis and phenol chloroform extracting methods. The results of features analysis indicate that the library consists of 30000 clones; the length of insertion element is between 30-45kb, and the average length is about 40kb; the total size of the fosmid library is estimated to 1171Mb. The insert DNA was stable during the propagation in the fosmid system. The results of end-sequencing indicate that the percentage of the M.incognita sequence was 2.04%, the bacterium sequence was 44.90%, the sequences without significant similarity was 53.06%; Sphingopyxis and Delftia were dominant communities of M.incognita associated bacteria.