Abstract:Three Triticum aestivum-Haynaldia villosa translocation lines were inoculated with eight dominant races (or single spore strains) of Puccinia striiforms f.sp. tritici prevalent in China. Three translocation lines, susceptible cultivar Mingxian 169 and their F1, F2, BC1F1 cross combinations and F2 generations of set dialleds crosses among these three translocation lines were tested with races CYR30, CYR31, Su-4 and the single spore strains CYR32-6, respectively, allelism tests of these three translocation lines were also done. It showed that three translocation lines were excellent resource resistance. The V9128-1 resistance to CYR30, CYR32-6 and Su-4 were all controlled by one dominant gene, resistance to CYR31 were controlled by one dominant gene and one recessive gene, independently, single dominant gene conferring resistance to these four dominant races (or single spore strains) in V9128-3, two complementary dominant genes conferring resistance to both CYR32-6 and Su-4, one dominant gene and one recessive gene conferring independently or single dominant gene to confer resistance to CYR31 in V3; V9128-1 and V9128-3 contain same or tightly linked genes conferring resistance to CYR31, CYR32-6 and Su-4, V9128-1 and V9128-3 contain different genes conferring resistance to CYR30, CYR32-6 and Su-4 to V3, and contain one same or tightly linked gene confer resistance to CYR31.