---

期 刊 ：植物保护学报 2011年

关键词：稻瘟病菌；DNA指纹分析；遗传宗谱；致病型；

Keywords:Magnaporthe grisea, DNA fingerprinting, genetic lineage, pathotype,

摘 要 ：为了探寻稻瘟病菌无性世代DNA水平的变异,明确江西省稻区稻瘟病菌遗传宗谱与致病型之间的对应关系,利用rep-PCR(repetitive element-based PCR)分子指纹分析技术,对稻区稻瘟病菌的群体结构和遗传多样性进行分析,并用41株代表性菌株对35个水稻品种进行了致病性测定。结果表明,以相似度75%为界,可以将不同稻区采集的99个菌株划分为14个遗传宗谱,其中,宗谱4、1和10为优势宗谱,分别包含37、18和12个菌株,占总数的37.37%、18.18%和12.12%;稻瘟病菌遗传宗谱与致病型间存在复杂的关系,同一宗谱的菌株对应多个致病型,而同一致病型的菌株,分属于不同的遗传宗谱,两者之间不存在简单的对应关系。

Abstract:In order to reveal the relationship between genetic lineages and pathotypes of Magnaporthe grisea as well as the variability of the fungus at DNA level, 99 isolates of M.grisea from rice growing area in Jiangxi Province were assessed for DNA polymorphism using the rep-PCR (repetitive element-based PCR) technique, and 41 isolates representing various genetic lineages were inoculated on 35 rice cultivars. The results showed that, based on the similarity of fingerprinting, 99 isolates in this study were classified into 14 genetic lineages (GL), in which GL4, GL1, and GL10 were the predominant lineages. The relationship between genetic lineages and pathotypes of M.grisea in Jiangxi was complicated. The same genetic lineage might contain several different pathotypes, while the same pathotype might distribute in different genetic lineages. No certain corresponding relationship was found between genetic lineages and pathotypes.

---