Abstract:In order to get a strong effect biocontrol bacteria for tobacco black shank, 347 bacteria strains were isolated from 30 soil samples, which collected from healthy tobacco rhizospheric soil. FB-16, a strain with strong effect on Phytophthora nicotianae, its inhibition zone was up to 23 mm. The methods of inhibition rate of mycelium growth, antifungal mechanism experiment, bonsai experiment in greenhouse were used to determine its antifungal effect. This strain was identified based on morphological, physiological and biochemical characteristics, and 16S rDNA phylogenetic analysis. The results showed that the inhibition rate of FB-16 fermented liquid against the mycelium growth of P.nicotianae was 95.96%. The FB-16 metabolite showed teratogenic effects on mycelia of P.nicotianae. The strain FB-16 was identified as Bacillus amyloliauefaciens (GenBank accession JN245982). The antifungal metabolite from stain FB- 16 extracted by 25% ammonium sulfate showed the most effective inhibition toward P.nicotianae, and its inhibition zone diameter was up to 42.00 mm. In the 7th day after treated with FB-16, the control effect on tobacco reached 69.96%. Conclusively, it has potential value in biological control against tobacco black shank.