Abstract:Leaf-dip, artificial soil and direct absorption methods were used conducted to determine the laboratory toxicity of six insecticides to the black cutworm, Agrotis ypsilon (Rottemberg), the acute response to the earthworm, Eisenia foelida and the effects on respiration of soil microorganism, respectively. Results showed that the toxicity of chlorfenapyr, indoxacarb and emamectin benzoate was significantly higher than that of chlorpyrifos, phoxim and lambadacyhalothrin. The acute response to the earthworm results showed that the LC50 of chlorfenapyr, indoxacarb and emamectin benzoate was 1 320.80, 1 153.42 and 167.91 mg/kg, which belonged to the inferior toxic category. The selective toxicity of chlorfenapyr was the highest among 6 insecticides tested, and its selective toxicity ratio reached 7 115.23. These pesticides all belong to the lower poisonous pesticide category on soil microbe.