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期 刊 ：植物保护学报 2010年

关键词：蚕豆萎蔫病毒2；黄瓜花叶病毒；系统进化树；

Keywords:Broad bean wilt virus 2, Cucumber mosaic virus, phylogenetic tree,

摘 要 ：为明确北京地区发生的地黄花叶病的病原,在进行生物学接种分离纯化的基础上利用RT-PCR方法对其进行了分子鉴定。通过接种指示植物和进行单斑分离,获得了病毒的纯分离物。经RT-PCR和序列测定及分析,有明显花叶症状的北京地黄样品受黄瓜花叶病毒Cucumber mosaic virus(CMV)和蚕豆萎蔫病毒2 Broad bean wilt virus 2(BBWV2)复合侵染。为进一步明确BBWV2地黄分离物(BBWV2-Rg)的分类地位,克隆了BBWV2-Rg RNA2多聚蛋白基因,并进行了序列测定和分析,结果表明该多聚蛋白基因由3195个核苷酸组成,编码1064个氨基酸。经序列比对分析,BBWV2-Rg编码的外壳蛋白大亚基LCP基因和小亚基SCP基因核苷酸序列与已发表的BBWV2其它株系相应基因核苷酸序列的同源性分别为78.69%~89.30%和76.99%~90.52%,氨基酸序列同源性分别为91.29%~97.51%和87.82%~96.45%。

Abstract:To identify the viruses that induced Rehmannia glutinosa mosaic disease in Beijing, biological inoculation and RT-PCR used. Virus isolates were purified through single lesions and propagated on indicator plants. Results of RT-PCR and sequencing showed that there were Cucumber mosaic virus (CMV) and Broad bean wilt virus 2 (BBWV2) in mosaic R.glutinosa leaves. To further characterize the BBWV2 isolate R.glutinosa (BBWV2-Rg), the polyprotein gene coded by BBWV2-Rg RNA2 was cloned and sequenced, and it consisted of 3195 nucleotides (nt), which encoded 1064 amino acid (aa) residues. Sequence alignments showed that BBWV2-Rg LCP and SCP, in nucleotide identity, were from 78.69% to 89.30% and from 76.99% to 90.52% to that of other BBWV2 strains, respectively, and in deduced amino acid, were from 91.29% to 97.51% and from 87.82% to 96.45% separately identical to that of other BBWV2 strains.

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