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期 刊 ：植物保护学报 2009年

关键词：海藻糖酶；高效氯氟氰菊酯；亚洲玉米螟；汰选；

Keywords:trehalase, lambda-cyhalothrin, Ostrinia furnacalis, pesticide selection,

摘 要 ：为评价亚洲玉米螟对高效氯氟氰菊酯的抗性风险,室内采用毛细管点滴处理4龄幼虫连续汰选11代,并采用葡萄糖氧化酶终点法测定了汰选品系F₁₀代试虫卵、不同龄期幼虫、蛹以及5龄幼虫不同组织部位海藻糖酶活性。结果表明,保持存活率约50%条件下用高效氯氟氰菊酯连续汰选11代,LD₅₀值为0.043μg/虫,除F₃代(LD₅₀ 0.086μg/虫)外,其它世代与F₀代(LD₅₀ 0.045μg/虫)差异不显著;汰选品系卵、3龄、4龄幼虫及蛹的海藻糖酶活性为14.54、31.42、53.28和30.79mU/mg pro.,明显低于对照;5龄幼虫的血淋巴和中肠组织中海藻糖酶活性分别为8.05和140.97mU/mg pro.,明显低于对照的58.86和316.17mU/mg pro.。

Abstract:In order to evaluate the resistance of Asian corn borer, Ostrinia furnacalis (Guene), to lambda-cyhalothrin, the successive selection keeping about 50% insects alive by topical application has been carried out for 11 generations. And the activities of trehalase in different instars and different tissues of the fifth instars were tested with the method of glucose oxidase. It is shown that the LD₅₀ of the F₁₀ generation is 0.043μg/individual. There is no significant difference in LD₅₀ between the control F₀ (0.045μg/individual) and any other generation, except the F₃ generation with the LD₅₀ of 0.086μg/individual. The activities of trehalase of egg, the 3^rd larvae, the 4^th larvae and pupa were 14.54, 31.42, 53.28 and 30.79mU/mg pro., significantly lower compared to those in the control. Moreover, the activities of trehalase in blood and midgut of the 5^th larvae were 8.05mU/mg pro. and 140.97mU/mg pro. significantly lower than the control.

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