---

期 刊 ：植物保护学报 2010年

关键词：西兰花；头腐病；病原鉴定；荧光假单胞菌；

Keywords:broccoli, head rot, pathogen identification, Pseudomonas fluorescens,

摘 要 ：为明确近年来浙江省宁波市部分地区发生的西兰花花球头腐病的病原,通过田间症状观察、致病性测定、Biolog和脂肪酸(fatty acid methyl ester, FAME)分析将分离到的病原菌鉴定为荧光假单胞菌Pseudomonas fluorescens。利用16S rDNA和核糖体DNA内转录间隔区(internal transcribed spacer, ITS)序列构建的系统发育树也都显示分离的西兰花菌株与假单胞菌聚合成群,其中与荧光假单胞菌的亲缘关系最近,序列同源性分别为99%和98%。表明浙江省西兰花花球上发生的细菌性头腐病由荧光假单胞菌引起。

Abstract:In order to elucidate the causal agent of broccoli head rot in Ningbo, Zhejiang Province, the causal bacterium was isolated and characterized with the pathogenicity test, Biolog and FAME (fatty acid methyl ester) test. The results indicated that the causal agent belong to Pseudomonas fluorescens. The Ningbo strains were most closely related to P.fluorescens in the phylogenetic trees constructed based on the 16S rDNA gene and 16S-23S rDNA internal transcribed spacer (ITS) gene of the Ningbo strains and related species, respectively, while the 16S rDNA and ITS gene sequences of the Ningbo strains had 99% and 98% similarity with that of P.fluorescens, respectively. Overall, this result showed that P.fluorescens is the causal agent of broccoli head rot.

---