Abstract:To develop an efficient, stable and easy method for disease evaluation, excised 2-years old twigs, current-year shoots, expanded leaves and mature fruits of ‘Fuji’ apple tree were inoculated with the plugs of isolate Slq0803-1-1-1. The results showed that lesions only occurred on the samples inoculated by the fungus with wound; samples with/without wound and inoculated with plain PDA plug were lesion free. The effects of materials and types of wound on lesion size presented that lesions formed on the twigs which treated by burning before inoculation were smaller and formed later than others; lesions on the young shoots with one pricking wound were bigger than those on the leaf scars; leaves were easier to be infected by pricking one time on the upper sides than lower ones even with ten times pricking wounds; lesions on fruits inoculated after peeling were bigger than treated with pricking. Furthermore, to verify the stability of the laboratory-test evaluation system, four isolates with different virulences were tested through inoculating on both excised materials and shoots in the field. The results showed that all the four excised materials could present the differences in virulence among strains, and the conclusions were consistent with field inoculation on shoots without wound. Therefore, all these four excised materials could be used to evaluate the pathogenicity of Botryosphaeria dothidea accurately and rapidly for laboratory-test, among which the excised leaf treated by one-time-pricking on the upper side before inoculation was the best option.