Abstract:Stripe rust caused by Puccinia striiformis f.sp. tritici is one of the most important wheat diseases in many regions of world including China. So far, breeding for resistance cultivars has been proved the most economic and effective approach for the control of this disease. A wheat line P81 showed excellent resistance to stripe rust isolates CYR30,CYR31 and CYR32 at seedling and adult stage. In this paper, F1, F2 and BC1, BC2 seeds were respectively prepared by crossing Chuanmai28 and Taichung29, two highly susceptible varieties, as female parent and P81 as male parent. Resistance of P81 and its two hybrid progenies were analyzed genetically by artificial inoculation with CYR32 at seedling stage. High resistance of two F1 plants to the race CYR32, similar to that of P81, indicated that the resistance gene in P81 was dominant. Based on the data of resistance phenotypes of F1, F2, BC1 of Chuanmai28/P81 and their parents by inoculated CYR32, it was showed there was a dominant gene in P81. While, the same result was gotten from the populations F2, BC2 in Taichung29/P81 inoculated CYR32. By allelic test, it indicated that the resistance gene in P81 was different from Yr5, Yr10, Yr15 and Yr26. Through pedigree analysis, it showed that the resistance gene in P81 derived from Syria common wheat line Xu29. These results suggested that the line P81 as a resistance resource had a considerable value in wheat breeding for stripe rust.