Abstract:Virgilio, one of the wheat cultivars used to differentiate races of Puccinia striiformis West. in China, was crossed with susceptible cultivar Mingxian 169 and cultivars with different genes to determine the genetic basis of its stripe rust resistance. Seedlings of the parents, F1, F2, BC1 and some F3 progenies were evaluated for resistance to Chinese pathotypes of P.striiformis.It was indicated that Virgilio has one recessive gene for resistance to Chinese pathotype CY17 and CY23, and one dominant gene for resistance to pathotype Su-1. When inoculated with CY17 or CY23, 48 F3 families of cross Mingxian 169/Virgilio could be separated into three groups and fit the 1 ∶ 2 ∶ 1 of theoretical ratio, this result inferred that Virgilio have any minor genes for resistance to CY17 and CY23 except for one major gene. Based on the results of linkage analysis, the gene that confers resistance to CY17 and CY23 was allele or closely linked to one gene in Triticum spelta album and was denominated as YrVir1. The gene that confers resistance to Su-1 was denominated as YrVir2.