Abstract:The samples of Colletotrichum gloeosporioides of mango in different orchards were collected and their resistance to carbendazim was tested in vitro. The results showed that the minimal inhibitory concentration(MIC) of sensitive isolates were 0.110μg/mL. However, resistant isolates were able to grow well even on 500μg/mL carbendazim. The resistance to carbendazim was stably inherited after continuously transferred for 10 generations on carbendazim-free PDA medium. Biological characters of resistant and sensitive isolates were tested and compared. The correlation analysis of MIC to no. of conidia produced and pathogenicity were carried out and the results showed that these factors were appeared no correlation of resistance to carbendazim. Resistant isolates showed positive cross-resistance to thiophanate-methyl, benomyl, thiabendazole and sensitivity to diethofencarb and prochloraz. The resistant and sensitive isolates were inoculated on mango fruits which were soaked in carbendazim solution in vitro. As a result, the fruits treated with fungicide and then inoculated with resistant isolates still showed disease symptoms as severe as the control, while the fruits inoculated with sensitive ones show no symptoms, which was response to resistant level of the isolates were tested on the PDA medium containing carbendazim. The tests on PDA medium can be used for supervising resistant isolates in the field.