Abstract:Kangyin 655, used to differentiate races of Puccinia striiformis West. in China, was crossed with stripe rust susceptible cultivar Mingxian 169 and cultivars with different genes for resistance to the disease to determine the genetic basis of its stripe rust resistance. Seedlings of the parents, F1, F2, and BC1 progeny were evaluated for resistance to Chinese pathotypes of P.striiformis. It was showed that Kangyin 655 has two genes for resistance to tested Chinese pathotypes, one determine the resistance to pathotypes CY29, the other, which confers resistance to CY29, CY31 and Su-1, is allele or closely linked to the gene in Triticum spelta album. Therefore it was proposed them as YrKy1 and YrKy2, respectively.