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期 刊 ：植物保护学报 2007年

关键词：小麦；遗传分析；分子标记；简单重复序列；

Keywords:wheat, genetic analysis, molecular marker, SSR,

摘 要 ：以小麦优异抗源Sw92为父本,感病小麦品种铭贤169为母本,杂交获得F₁、F₂和BC₁代群体。采用我国叶锈菌优势小种PHT对双亲及其杂交世代进行接种鉴定。结果表明,小麦抗源Sw92对叶锈菌小种PHT的抗性系由一对隐性基因所控制。采用简单重复序列(SSR)技术对Sw92携带的抗性基因进行分子标记,共筛选了371对SSR引物,获得2个引物(WMC494、WMC737)可在抗/感池和双亲中扩增出多态性DNA片段。遗传连锁分析结果表明,该抗病基因位于小麦6BS上,与WMC494、WMC737标记的遗传距离分别为3.4cM和15.0cM,不同于6BS上的已知抗叶锈基因Lr36和Lr53, 暂命名为LrSw92。

Abstract:Wheat line Sw92 is highly resistant to Puccinia triticina in China either at seedling stage or adult plant stage. F₁, F₂ and BC₁ generations from the crossing of Mingxian 169×Sw92 was inoculated with the dominant race PHT of Puccinia triticina. The gene for leaf rust resistance of Sw92 was detected by the genetic analysis. The results indicated that resistance of Sw92 to the race PHT of Puccinia triticina was controlled by one pair of recessive genes. The bulked segregant analysis (BSA) of F₂ generations and simple sequence repeats (SSR) techniques were used to develop molecular markers linked to the resistance gene in Sw92. The resistant and susceptible DNA pools were prepared separately from the F₂ plants crossing between Mingxian169 and Sw92. Two SSR markers namely WMC494 and WMC737 on 6BS of wheat chromosome closely linked to the leaf rust resistance gene was obtained. The results of linkage analysis showed that the gene for leaf rust resistance in Sw92 was located on 6BS with the genetic distance of 3.4cM to WMC494 and 15.0cM to WMC737, respectively, which probably is a new leaf rust resistance gene LrSw92(temporary designation)other than the known Lr36 and Lr53 on 6BS of wheat.

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